毒物代谢酶CYP2E1基因多态性与慢性苯中毒易感性的关系  

The relationship between gene polymorphism of toxin metabolic enzyme CYP2E1 and susceptibility to chronic benzene poisoning

作　　者：叶榕[1] 管继如[1] 谢晓敏[2] 夏昭林[3] 罗建红[4] 张国军[5] 潘劲草[1] 万俊香[3] HAN Chen-long 

机构地区：[1]杭州市疾病预防控制中心,浙江,杭州,310006 [2]杭州市红十字会医院,浙江,杭州,310004 [3]复旦大学公共卫生学院,上海,200032 [4]浙江大学医学院,浙江,杭州,310006 [5]杭州市卫生监督所,浙江,杭州,310004 [6]Cancer Institute,National Institutes of Health,Bethesda,Maryland,USA

出　　处：《中国工业医学杂志》2003年第3期143-147,共5页Chinese Journal of Industrial Medicine

摘　　要：目的　探讨CYP2E1基因多态性与苯中毒遗传易感性的关系。方法　选取苯中毒组和苯接触组工人共 14 2名 ,进行健康及生活方式 (吸烟、饮酒等 )调查、内科体检、外周血常规检验 ;PCR扩增基因DNACYP2E15′端上游调节区RsaI、PstI位点以及第六内含子区DraI位点片断 ,限制性内切酶检测基因多态性 ;现场空气苯浓度测定。结果　苯中毒组男性携带c1/c2基因型者患慢性苯中毒的危险性为c1/c1型的 3 5倍 ( 95 %CI 1 0 10～ 12 382 ) ,携带CD基因型者患慢性苯中毒的危险性是DD基因型的 4 0倍 ( 95 %CI 1 70 4～ 15 374)。男性携带c1/c2或CD联合基因型患慢性苯中毒的危险性为c1/c1+DD联合基因型的 7 8～ 13倍。苯中毒组接苯工龄高于苯接触组 ,防护措施状况差于接触组 ,差异有显著性。饮酒、吸烟对CYP2E1有诱导或促进作用 ,本次观察尚未发现两组差异有显著性。结论　慢性苯中毒发生是基因 环境等多种因素作用的结果 ,男性携带c1/c2和CD基因型者发生慢性苯中毒的相对危险度增大 ,可能与性别、遗传、生理状况及生活方式等多种因素有关。苯作业场所的防护措施对工人的保护作用相当重要 ,接苯工龄越长 。Objective To explore the relationship between gene polymorphism of CYP2E1 and individual susceptibility to benzene poisoning.Method 142 subjects were investigated,including benzene intoxication group and benzene exposed group.Except routine physical examination and blood routine test,analysis on gene polymorphism of CYP2E1 at 5′-flanking region RsaI/PstI and intron-6 DraI,concentrations of benzene in the air of workplace were also measured.Result Gender stratification analysis showed that male worker with c1/c2 genotype showed 2.5(95% CI 1.010～12.382)fold higher risk suffering from benzene poisoning than that of male with c1/c1 genotype;and male workers with CD genotype had a 3.0(95%CI 1.704～15.374)fold higher risk for benzene intoxication than that of whose with DD genotype.Male workers with either c1/c2 or CD genotypes showed a 6.8～12 fold higher risk for benzene intoxication than that with c1/c1+DD genotype.Conclusion The occurrence of chronic benzene poisoning is the result from various agents including gene polymorphism of metabolic enzymes,working environment,benzene exposure level,exposure time,gender and life-styles etc.Male with either c1/c2 or CD genotypes might have higher risk suffering from benzene poisoning than others.

关 键 词：毒物代谢酶 CYP2E1 基因多态性 苯中毒 遗传易感性 

分 类 号：R135.12[医药卫生—劳动卫生]