酶免疫组化法对肾综合征出血热早期诊断的方法学研究  

THE METHODOLOGY STUDIES ON THE HISTOCHEMICAL ASSAY DIAGNOSIS OF HIMRRHAGIC FEVER IWTH RENAL SYNDROME BY IMMUNO-ENZYMATIC

作　　者：李闻文[1] 吴水河[2] 阎祖炜[1] 龚道科[1] 戴敢[1] 

机构地区：[1]中南大学湘雅医学院检验系临床微生物学免疫学教研室,长沙410078 [2]卫生部肝胆肠外科研究中心

出　　处：《中国现代医学杂志》2003年第13期65-67,共3页China Journal of Modern Medicine

摘　　要：目的 :用酶免疫组化法对肾综合征出血热进行早期诊断。方法 :将肾综合征出血热病毒 (HFRSV)接种于Vero -E6细胞 ,培养后制高效价病毒抗原片。应用酶免疫组化技术进行检测。结果 :酶免疫组化技术对肾综合征出血热能早期、快速、特异性地诊断。结论 :实验证明该法具有早期诊断、快速简便、特异敏感等特点 ,其检测结果可长期保存等优点 ,尤其适用于基层医疗单位推广应用。Objective:The early diagnosis of hemorrhagic fever with renal syndrome by immuno-enzymatic histochemical assay. Methods:Hemorrhagic fever with renal syndrome virus(HFRSV) were inoculated with Vero-E6 cell, after culture, the high titer virus antigen slides were made for using to immuno-enzymatic histochemical assay.Results:It is superior in early rapid, specifivity, sensitivity diagnosis. Conclusions:The immuno-enzymatic histochemical assay is superior in early rapid, specifivity, sensitivity and simplicity, long time to store. This method is suitable for basic hospital.

关 键 词：肾综合征出血热 酶免疫组化技术 诊断 

分 类 号：R692[医药卫生—泌尿科学]