益气活血法对心肌缺血再灌注损伤大鼠肿瘤坏死因子-α及丙二醛含量的影响  

Effect of Qi-supplementing and Blood-activating Therapy on the Levels of TNFα and MDA in Rats with Ischemia Reperfusion Damage

作　　者：戴小华[1] 韩宁林[1] 周宜轩[1] 俞浩[1] 徐先祥[1] 顾健霞[1] 汪玲[1] 丁碧云[1] 

机构地区：[1]安徽中医学院第一附属医院,安徽合肥230031

出　　处：《安徽中医学院学报》2003年第4期39-41,共3页Journal of Anhui Traditional Chinese Medical College

基　　金：安徽省教育厅自然科学研究项目 (2 0 0 1KJ15 7)

摘　　要：目的 :观察益气活血法对心肌缺血再灌注大鼠肿瘤坏死因子 α(TNFα)及丙二醛 (MDA)含量的影响 ,探讨其抗心肌缺血再灌注损伤的作用机制。方法 :选用Wistar大鼠 ,随机分为假手术组、心肌缺血组 (缺血组 )、心肌缺血再灌注组 (再灌注组 )和再灌注治疗组 (治疗组 ) ,治疗组每日灌胃中药 (益气活血方 ) 1次 ,连续 5d ;其余 3组每日灌胃等量 9.0 g/L氯化钠注射液 1次 ,连续 5d ,然后复制模型并测定血清TNFα及MDA含量。结果 :结扎大鼠左冠状动脉前降支 5min时 ,各组心电图与假手术组相比 ,ST段 (J点 )抬高 (P <0 .0 1) ,T波高耸 (P <0 .0 1) ,治疗组ST段抬高显著低于缺血组及再灌注组 (P <0 .0 1) ;各组TNFα及MDA含量与假手术组相比均升高 ,治疗组显著低于再灌注组 (P <0 .0 5 )。结论 :益气活血法能够减轻大鼠心肌缺血再灌注损伤 ,可能与减少心肌细胞释放肿瘤坏死因子。Objective:To study the effect of Qi-supplementing and Blood-activating Therapy on the levels of tumor necrosis factor-α(TNFα) and malonyldialdehyde (MDA), and explore the mechanism of Qi-supplementing and Blood-activating Therapy against ischemia reperfusion damage in rats.Method:Animal model of ischemia reperfusion was established. The levels of TNFα and MDA in serum were measured. Result: Compared with control group, the levels of TNFα and MDA increased in treatment group and ischemia reperfusion group. TNFα and MDA in treatment group significantly decreased compared with those of ischemia reperfusion group. Conclusion: The effect of Qi-supplementing and Blood-activating Therapy against rat cardiac muscel cells injury may associate with decreasing the level of TNFα released by cardiac muscle cells,scavenging oxygenic radicals.

关 键 词：心肌缺血 再灌注损伤 益气活血法 大鼠 肿瘤坏死因子-α 丙二醛 中医药疗法 

分 类 号：R259.422[医药卫生—中西医结合]