冠心病家族史大学生apo E基因多态性的临床研究  

Relationship between apolipoprotein E gene polymorphism and atherosclerosis and coronary heart disease in college students

作　　者：李洁[1] 张天民[2] 李莎 詹如冰[4] 林清霞[1] 黎明[1] 

机构地区：[1]广东工业大学医院心内科,510090 [2]武汉科技大学医学院,430080 [3]广州市东山人民医院,510080 [4]广东省人民医院,510080

出　　处：《国外医学（心血管疾病分册）》2003年第4期248-250,共3页

基　　金：广东工业大学立项资助课题;项目编号:202002

摘　　要：目的:探讨大学生载脂蛋白E(apoE)基因多态性,及其对血脂代谢紊乱、动脉粥样硬化(AS)和冠心病(CHD)的影响。方法:对广东地区高校152名健康汉族大学生,在问卷调查的基础上,根据一、二级亲属有无CHD病史分为阳性组与对照组,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法进行apoE基因分析。结果:有CHD家族史的阳性组e_4等位基因频率显著高于无CHD家族史的阴性组(P<0.05)。结论:apoE基因多态性与AS、CHD易感性密切有关,e_4可能是CHD的重要遗传因子。Objective: To investigate the effect of apolipoprotein E(apo E) gene polymorphism on blood lipids metabolism and the incidence of atherosclerosis (AS) and coronary heart disease (CHD) in college students . Methods: One hundred fifty two healthy students of Han nationality in the universities of Guangdong region were investigated by using polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) analysis. With the help of information from questionnaire, they were divded into 2 groups according to whether or not they had positive CHD family history and then they underwent apoE genotype analysis. Results: The allele frequency of apo e4 in positive group was significantly higher than that of negative group (P<0.05). Conclusions: The apo E gene polymorphism correlated significantly with susceptibility to AS and CHD, and e4 may be an important genetic factor of CHD.

关 键 词：冠心病 家族史 载脂蛋白E 基因多态性 动脉粥样硬化 血脂代谢紊乱 聚合酶链反应-限制性片段长度多态性 

分 类 号：R541.4[医药卫生—心血管疾病] R394[医药卫生—内科学]