血清骨钙素N端中分子片段的测定及临床应用  

Quantitative determination of serum N-MID osteocalcin and its clinical application

作　　者：甘洁民[1] 张月丽[1] 施泓[1] 汪家愉[1] 缪应新[1] 

机构地区：[1]上海市华东医院,上海200040

出　　处：《上海医学检验杂志》2003年第4期216-218,共3页Shanghai Journal of Medical Laboratory Sciences

摘　　要：目的　研究各年龄组血清骨钙素N端中分子 (N MID)片段的含量 ,了解其稳定性 ,并探讨与各种骨代谢疾病的关系。方法　用电化学发光免疫测定法 (ECLIA)测定血清骨钙素N MID片段 ,其中对照组 183例 ,各种骨代谢疾病患者 99例。将样品放置于室温和 4℃中 ,于不同时间分别测定血清骨钙素N MID片段和全段骨钙素。结果　女性绝经后含量显著升高 ,男性各年龄组差异无统计学意义 ,女性高于男性 ,各类疾病组与对照组比较差异均有显著性。样品在室温和 4℃冷藏 ,骨钙素N MID片段很稳定 ,而全段骨钙素逐渐下降。结论　用ECLIA法测定血清骨钙素N MID片段 ,灵敏度高 ,稳定性 ,能很好地反映骨转换的变化 ,是骨代谢疾病研究非常有价值的敏感指标。Objective To study the N-MID osteocalcin in different ages, investigate the stability of osteocalcin, and study the relationship between N-MID osteocalcin and bone metabolic disease. Methods N-MID osteocalcin in human serum was determined by electrochemiluminescence immunoassay (ECLIA). Blood samples were collected from 183 healthy subjects and 99 patients with bone metabolic disease. The stability of the N-MID osteocalcin was observed by comparison of storing samples in room temperature and 4 ℃. Results A significant increase of N-MID osteocaltin was found in postmenopausal women. The serum N-MID osteocalcin in women is higher then in men. A significant increase of N-MID osteocalcin was found in bone metabolic disease. The N-MID osteocalcin is stable in room temperature and 4 ℃, but the intact osteocalcin decreases significantly. Conclusions ECLIA for quantitative determination of N-MID osteocalcin can improve the clinical utility and be useful to assess bone metabolic disease.

关 键 词：骨钙素 N端中分子片段 电化学发光免疫测定法 骨代谢疾病 

分 类 号：R681[医药卫生—骨科学]