头孢克洛和δ-3-头孢克洛的毛细管区带电泳分离  

Separation of Cefaclor andδ-3-Cefaclor by Capillary Zone Electrophoresis

作　　者：贾丽[1] 周维[1] 

机构地区：[1]汕头大学理学院化学系分析测试中心,广东汕头515063

出　　处：《分析测试学报》2003年第4期55-57,共3页Journal of Instrumental Analysis

基　　金：汕头大学校基金资助项目 (130-512040)

摘　　要：建立了分离头孢克洛和δ_3_头孢克洛的毛细管区带电泳法 (CZE) ;在CZE中 ,以4_磺酸杯芳烃[6]作为异构体选择性试剂成功地分离了头孢克洛和δ_3_头孢克洛 ;当以4_磺酸杯芳烃[6]作为异构体选择性试剂时 ,背景缓冲液的pH值和4_磺酸杯芳烃[6]的浓度是影响头孢克洛和δ_3_头孢克洛分离的重要参数 ;同时还考察了有机溶剂和 β_环糊精对分离的影响 ;并将该方法成功地用于测定口服头孢克洛干混悬剂中的有效成分———头孢克洛 。A method,capillary zone electrophoresis,for the separation of cefaclor andδ_3_cefaclor was deve_ loped.In the method,4_sulfonic calixarene(SCX6)was used as an isomer separating agent.The concentration of SCX6and pH of the background buffer were found to be important parameters in the separation of cefaclor andδ_3_cefaclor.The influences of organic modifiers andβ_cyclodextrin on the separation were also investigated.The method was applied to separate and determine active ingredient in cefaclor for oral dry suspension successfully.

关 键 词：头孢克洛 δ-3-头孢克洛 毛细管区带电泳 分离 4-磺酸杯芳烃[6] 测定 口服头孢克洛干混悬剂 

分 类 号：R978.11[医药卫生—药品] R917[医药卫生—药学]