甲苯咪唑与牛血清白蛋白作用的荧光光谱特征  

Fluorescence spectral Characteristics of the Binding Reaction of Mebendazole with Bovine Serum Albumin

作　　者：颜承农[1] 上官云凤[1] 潘祖亭[2] 刘义[2] 

机构地区：[1]长江大学化学系,湖北荆州434020 [2]武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《分析测试学报》2003年第4期24-27,共4页Journal of Instrumental Analysis

基　　金：湖北省教育厅科研重点资助项目 (2003A003)

摘　　要：用荧光光谱法研究了甲苯咪唑与牛血清白蛋白结合反应的光谱特征 ,证实由于非辐射能量转换机制 ,甲苯咪唑对牛血清白蛋白有较强的荧光猝灭能力 ,按照Stern-Volmer方程和双倒数方程分析处理实验数据 。The spectral feature of binding reaction of mebendazole with bovine serum albumin(BSA)was studied by fluorescence spectroscopy.It was shown that mebendazole has a powerful ability to quench the BSA fluorescence via a nonradiative energy transfer mechanism.The fluorescence quenching data were analyzed according to Stern-Volmer equation and double-reciprocal equation,and the binding constants,and the thermodynamic parameters were obtained.

关 键 词：甲苯咪唑 牛血清白蛋白 作用 荧光光谱 特征 荧光猝灭 热力学参数 驱肠虫药 作理作用 

分 类 号：R978.6[医药卫生—药品] R962[医药卫生—药学]