利用核核糖体DNA内转录间隔区(ITS)探讨广义羽藓科系统发育  

Using Nuclear Ribosomal DNA Internal Transcribed Spacers (ITS) to Discuss the Phylogeny of Thuidiaceae(S.L.,Musci)

作　　者：张会[1] 黄勤妮[1] 杜桂森[1] 吴鹏程[2] 刘霞[1] 

机构地区：[1]首都师范大学生物系,北京100037 [2]中国科学院植物研究所,北京100093

出　　处：《云南植物研究》2003年第4期491-496,共6页Acta Botanica Yunnanica

基　　金：北京市自然科学基金资助项目

摘　　要：利用核核糖体DNAITS序列 ,探讨了苔藓植物广义羽藓科的系统发育 ,摸索出适于扩增ITS片段的最适反应条件。实验共得到广义羽藓科 6个种的ITS序列 ,它们分别是 :Abieti nellaabietina (AJ4 174 94 ) ,Anomodonminor (AJ344 14 5 ) ,Claopodiumaciculum (AJ315 96 8) ,Thuidiumpristocalyx (AJ4 16 443) ,Thuidiumassimile (AJ4 16 442 ) ,Herpetineurontoccoae(AJ315 96 7) ,其中后 5个种是国际上首次得到的。本文利用ITS序列构建羽藓科 7属、 11种植物的系统发育树 ,据Bootstrap严格一致树表明 :广义的羽藓科为并系发育 ,可分为两个主要的分支 ,牛舌藓属Anomodon ,羊角藓属Herpetineuron和多枝藓属Haplohymenium等为一支 ,而山羽藓属Abietinella ,羽藓属Thuidium ,沼羽藓属Helodium和麻羽藓属Claopodium等为另一主要分支 ,从分子水平上支持了据形态特征把原牛舌藓亚科的牛舌藓属 ,羊角藓属 ,多枝藓属提升为牛舌藓科的结论。In this paper, we analyzed the phylogenetic relationship of Thuidiaceae (s.l., Musci) using nuclear ribosomal DNA internal transcribed spacers (ITS),from which we got 6 species' ITS sequences of Thuidiaceae (s.l.), including Abietinella abietina (AJ417494), Anomodon minor (AJ344145), Claopodium aciculum (AJ315968), Thuidium pristocalyx (AJ416443), Thuidium assimile (AJ416442) and Herpetineuron toccoae (AJ315967), and then they were sequenced directly from PCR products. Among them, except Abietinella abietina ,the other five species' ITS sequences are all the first time to be reported in science. Finally we constructed the Bootstrap strict consensus trees of seven genera of Thuidiaceae (s.l.) which indicated that Thuidiaceae (s.l.) has two main paraphyletic clades, one including the genera Anomodon, Herpetineuron, Haplohymenium et al. , and the another one including Thuidium,Claopodium,Helodium et al .

关 键 词：核核糖体DNA 内转录间隔区 羽藓科 系统发育 

分 类 号：Q941[生物学—植物学]