禾谷镰孢菌β-微管蛋白基因克隆及其与多菌灵抗药性关系的分析  

Cloning of β-tubulin Gene from Gibberella zeae and Analysis its Relationship with Carbendazim-resistance

作　　者：李红霞[1] 陆悦健[1] 王建新[1] 周明国[1] 

机构地区：[1]南京农业大学农业部病虫监测与治理重点开放实验室,南京210095

出　　处：《微生物学报》2003年第4期424-429,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金 ( 3 0 0 70 5 1 0;3 0 2 0 0 1 81 ) ;教育部<高等学校骨干教师资助诸>项目~~

摘　　要：应用 3对引物 ,从禾谷镰孢菌 (Gibberellazeae)对多菌灵 (MBC)的敏感菌株 (MBCS)和田间及室内诱导抗药性菌株 (MBCR)中扩增 β 微管蛋白基因。该基因全长 1 631bp ,包含 3个内含子 ,编码 447aa ,与其他常见植物病原丝状真菌 β 微管蛋白基因的氨基酸同源性达 95 .1 2 %～ 99.30 %。MBCS 和MBCR 菌株核苷酸序列分析表明 ,MBCR 菌株未发生任何位点的突变 ,说明G .zeae对MBC的抗药性机制并非像其他丝状真菌一样由 β 微管蛋白 1 98位氨基酸突变所致。Whole β-tubulin genes from wild carbendazim(MBC)-sensitive isolate, field MBC-resistant isolate and induced MBC-resistant mutant of Gibberella zeae were cloned and sequenced with 3 pairs of primers. These genes have 1631bp length, including 3 introns, encoding 447 amino acid. The homology of amino acid tubulin gene of G. zeae with that of other common plant pathogenic filamentous fungi is from 95.12%～99.30%. Sequence comparison among MBC S, field MBC R and induced MBC R isolates revealed there was no mutation, even one. So it can be concluded that the mechanism of MBC-resistance to G. zeae is different from other filamentous fungi caused by point mutation at amino acid position 198 or other position of β-tubulin gene.

关 键 词：禾谷镰孢菌 β-微管蛋白基因 克隆 多菌灵 抗药性 小麦赤霉病 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]