H9N2亚型禽流感病毒基因组全长序列测定和各基因的遗传分析  

Genetic Characterization of the Entire Genome of an H9N2 Avian Influenza Virus A/Chicken/Shanghai/F/98

作　　者：卢建红[1] 刘秀梵[1] 邵卫星[1] 张评浒[1] 韦栋平[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,扬州225009

出　　处：《微生物学报》2003年第4期434-441,共8页Acta Microbiologica Sinica

基　　金：江苏省"十五"科技攻关项目 (BE2 0 0 2 0 1 6) ;农业部资助项目 (农办牧 2 0 0 1 83 )~~

摘　　要：用RT PCR技术及cDNA末端快速扩增法获得禽流感病毒分离株A Chicken Shanghai F 98(H9N2 )代表基因组全长的 8个基因片段。基因组序列比较及遗传进化分析结果表明 ,Chicken Shanghai F 98的 8个基因均不属于Quail HongKong G1 97亚系 ,与香港禽流感事件没有直接关系。它与Chicken Beijing 1 94的HA、NA、M、NS基因同源率分别为 96 7%、96 4%、97 5 %和 98 0 % ,这 4个基因属于Chicken Beijing 1 94亚系 ,其中 ,NA基因与Duck HongKong Y2 80 97的同源率为 97 4% ,而且它们均在 2 0 5位后缺失 9个核苷酸。而PB2、PB1、PA和NP基因与已知的 3个亚系关系较远 ,分别在相应的进化树上另成分支。因此 ,Chicken Shanghai F 98是两个以上不同基因亚系间发生自然重排的产物。Eight full-length genes of an H9N2 subtype avian influenza virus A/Chicken/Shanghai/F/98(H9N2), were amplified by RT-PCR and rapid amplification of cDNA ends (RACE) and sequenced. The results of phylogenetic analysis showed that A/Chicken/Shanghai/F/98 was entirely different from Quail/Hong Kong/G1/97 which was directly related to human infection with avian influenza virus in Hong Kong, whereas its HA, NA, M and NS genes belonged to A/Chicken/Beijing/1/94-like sublineage with the nucleotide homology of 96.7%, 96.4%, 97.5% and 98.0% respectively. The homology of NA gene between A/Chicken/Shanghai/F/98 and A/Duck/Hong Kong/Y280/97 was 97.4% and they both lost 9 nucleotides between nt 205 to 206. However, the PB2, PB1, PA, NP genes did not show any close relationship with those of the three known sublineages and the later three didn't share immediate ancestors with available isolates whose sequences had been reported. Therefore, A/Chicken/Shanghai/F/98 is a product of natural reassortment between H9N2 avian influenza viruses from different sublineages.

关 键 词：H9N2亚型禽流感病毒 基因组 序列测定 遗传分析 

分 类 号：S852.65[农业科学—基础兽医学]