薄层层析指导下的嗜碱放线菌菌株YIMGQ-14次生代谢产物研究  

The Secondary Metabolites Research of the Alkalophlic Strain YIMGQ14 Tracked by the TLC Assay

作　　者：李铭刚[1] Groth Ingrid 李一青[1] Sattler Isabel 文孟良[1] 

机构地区：[1]云南大学教育部微生物资源开放研究重点实验室,昆明650091 [2]Hans-Knll-Institute for Natural Product Research

出　　处：《微生物学报》2003年第4期481-486,共6页Acta Microbiologica Sinica

基　　金：国家科技部重点项目 ( 2 0 0 1CCC0 0 60 0 );云南省自然科学基金 ( 2 0 0 1C0 0 3R)~~

摘　　要：在微生物资源的开发研究过程中 ,利用TLC方法 ,配合TLC数据库的检索 ,对嗜碱放线菌菌株YIMGQ1 4的次生代谢产物进行了研究。并从中分离纯化出两个具有生物活性的专利化合物“UmycinC”和“UmycinB”。结果证明 :相对于常规的生物活性筛选 ,TLC法在微生物次生代谢产物研究中具有快速、简便、经济的特点 ,适合于小量常规筛选的目的。通过进一步对相关的TLC数据库进行构建和补充 ,将能极大地改善该方法的使用效率。In the course of the microbial resources exploitation, the secondary metabolites research of the alkalophilic strain YIMGQ-14 was carried on using the TLC method and with the coordinate of the TLC database searching. Two patented biological active compounds “Umycin C” and “Umycin B” were isolated and purified. The result showed that, compared with the ordinary biological screening, the TLC screening is fast, convenient, economical and much more suitable for the small scale daily screening purpose. With the reconstruction and supplement of the related TLC data, the working efficiency of this method can be improved greartly.

关 键 词：薄层层析 嗜碱放线菌 次生代谢产物 生物活性 放线菌 

分 类 号：TQ465[化学工程—制药化工]