咖啡酸对辐射损伤小鼠造血恢复作用的初步研究  

Preliminary Study on Hematopoietic Reconstruction Effects of Caffeic Acid in Lethally Irradiated Mice

作　　者：杜遵民[1] 贾海鹏[1] 刘静[1] 杨莉莉[1] 姜晓玲[1] 陈方国[1] 

机构地区：[1]解放军第八十八医院血液科,山东泰安271000

出　　处：《中国辐射卫生》2015年第5期449-452,共4页Chinese Journal of Radiological Health

基　　金：济南军区联勤部科研项目

摘　　要：目的观察咖啡酸对辐射损伤小鼠造血恢复的影响。方法建立接受4.5 Gy X射线照射的辐射损伤小鼠模型,BALB/c小鼠随机分为空白对照组,单纯照射组,CFA组,观察小鼠30天存活率、体重、外周血白细胞(WBC)、血红蛋白(HGB)、血小板(PLT)、骨髓有核细胞(BMNC)、粒细胞巨噬细胞集落形成单位(CFU-GM)、巨核细胞形成单位(CFU-MK)及脾集落形成单位(CFU-S)和脾脏指数(SI)等指标。结果 CFA组小鼠的30天存活率、体重、WBC、BMNC、CFU-GM、CFU-MK、CFU-S和SI数均明显高于单纯照射组,差异有统计学意义(P<0.05或P<0.01)。结论CFA能促进辐射损伤小鼠的造血恢复。Objective To investigate the hematopoietic reconstruction effects of caffeic acid( CFA) in lethally irradiated mice. Methods 42 BALB / c mice were randomly divided into 3 groups,14 rats in each group: normal control group( no radiation,no filling medicine),irradiation group( radiation,no filling medicine),CFA group( administered CFA after irradiation). The mice in all groups except normal control group were irradiated with 4. 5 Gy X-ray. The mice in CFA group were poured through gastic rote with CFA at the dose of 100 mg / kg,once perday for 28 days. The survival rate of 30 days,body weight,the number of white blood cells( WBC),hemoglobin( HGB),platelet( PLT),bone marrow nuclear cells( BMNC),granulocyte-macrophage colony-forming unit( CFU-GM),colony-forming unit of megakaryocyte( CFU-MK),colony-forming unit-spleen( CFU-S),spleen index( SI) were observed. Results Survival rate,body weight,WBC,HGB,PLT,BMNC,CFU-GM,CFU-MK,CFU-S,SI counts were significantly higher in CFA group than those of the irradiation group( P < 0. 01 or P < 0. 05). Conclusion Caffeic acid could effectively reconstruct hematopoiesis in lethally irradiated mice.

关 键 词：咖啡酸 辐射损伤 造血抑制 

分 类 号：R818[医药卫生—放射医学]