闽粤沿海鮸状黄姑鱼野生种群和人工繁育群体遗传多样性的RAPD分析  

GENETIC DIVERSITY OF NATURE AND REARED GROUP OF Nibea miichthioides BY RAPD ANALYSIS

作　　者：丁少雄[1] 苏永全[1] 王军[1] 全成干[1] 

机构地区：[1]厦门大学海洋学系

出　　处：《海洋科学》2003年第8期63-66,共4页Marine Sciences

基　　金：国家自然科学基金39870562号

摘　　要：采用RAPD技术对鮸状黄姑鱼(Nibea miichthioides)野生种群及人工繁育群体进行了DNA多态性检测。共采用40个随机引物进行扩增,其中35个引物在野生种群和人工繁育群体中都扩增出230个RAPD位点。野生种群和人工繁育群体的多态位点比率P分别为16.5％和15.7％;群体相似系数S为0.954和0.967;Shannon信息指数H_0为0.113和0.104;基因多样性指数H为0.085和0.083;而两群体间的遗传距离D为0.022。所得结果表明两群体间具有非常相近的亲缘关系,且遗传多样性水平都比较贫乏。The genetic diversity between wild population and reared group of Nibea miichthioides was investigated by RAPD analysis in present study. 35 decamer random primers screened from 40 Operon' s primer series were used to amplify RAPD fragments using a standard RAPD protocol. Total 230 DNA loci were detected in the two groups. The results showed that : (1) The ratio of polymorphic loci (P) and genetic similarity (S) of the wild and reared group of Nibea miichthioides were 16.5% 、0. 954 and 15.7% 、 0.967 respectively; (2) The Shannon's information index (Ho) and Nei's index were 0.113, 0.085 and 0.104, 0.083 for the two groups separately; (3) The genetic distance between the groups was 0.022. It indicated that the wild population of Nibea miichthioides had a close relationship with the reared group, and both of them possess of low genetic variation levels.

关 键 词：闽粤沿海 鮸状黄姑鱼 野生种群 人工繁育群体 遗传多样性 RAPD分析 

分 类 号：Q959.483[生物学—动物学] Q953