九株染料脱色菌的脱色特性及其质粒与基因分析  

Decolorizing Characterizations,Plasmids and 16S rRNA Gene Analysis of Nine Decolorizing Bacteria

作　　者：许玫英[1] 郭俊[1] 简浩然[1] 孙国萍[1] 

机构地区：[1]广东省微生物研究所

出　　处：《城市环境与城市生态》2003年第4期71-73,共3页Urban Environment & Urban Ecology

基　　金：广东省自然科学基金青年项目(000211);广东省自然科学基金研究团队项目资助(20015017)

摘　　要：对9株染料脱色菌的脱色特性及其质粒的携带情况进行初步的研究,测定和比较了这9株细菌的16SrDNA序列,并对其进行分子鉴定和分类,经检测的9株细菌均属于肠杆菌科,其中脱色能力较广谱的5株细菌均未检测到质粒的存在,它们在系统分类地位上基本上聚为一类,与埃希氏菌属的大肠埃希氏菌具有较高的同源性。而另外4株脱色能力单一、仅对三苯基甲烷类染料有较强脱色能力的菌株除一株以外,其它3株均携带有大分子量的质粒,在系统分类地位上也基本聚为另一类。The decolorizing characterizations,the plasmids and the nearly complete sequences of 16S rDNA from nine isolated bacteria were determined.The sequences were then used as molecular criteria to investigate the bacteria identities and their classification.The results show that the nine bacteria all belong to Enterobacteriaceae.The five bacteria with broad spectrum decolorizing ability were all not found plasmids.They were clustered together and showed high homogeneous with E.coli in the phylogenetic tree.Three of the four bacteria capable of decolorizing triarylmethane dye all harbored plasmids and showed close relationship in the phylogenetic analysis.

关 键 词：染料脱色菌 脱色特性 质粒 基因 DNA 环境污染 污染治理 

分 类 号：X788[环境科学与工程—环境工程]