转化生长因子β在静脉移植物内膜增生中的作用  

Effect of transforming growth factor-βon intimal hyperplasia of venous grafts

作　　者：萧明第[1] 尤文俊[1] 袁忠祥[1] 徐根兴[1] 

机构地区：[1]上海市第一人民医院心血管外科,上海200080

出　　处：《中国胸心血管外科临床杂志》2003年第3期184-187,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

摘　　要：目的　研究转化生长因子β(TGF-β)和血管紧张素 (ANG )在静脉移植物内膜增生中的作用及相互关系 ,为预防静脉移植物内膜增生寻找合理有效的途径。　方法　 2 0只新西兰大白兔按随机排列表法分为治疗组和对照组 ,每组 10只兔。两组兔均行颈外静脉 -颈总动脉移植术 ,治疗组术后给予 ANG 受体阻断剂洛沙坦 ,2 5 mg· kg- 1 / d,对照组给安慰剂治疗。应用半定量方法于术后 4周检测静脉移植物中 TGF-β、胶原 、胶原 m RNA水平。　结果　治疗组 TGF- β、胶原 、胶原 m RNA与内参照象素值比值明显低于对照组 (P<0 .0 1)。　结论　TGF- β和 ANG 在静脉移植物内膜增生中发挥着重要作用 ,ANG 与受体结合后直接导致 TGF-β m RNA上调 ,TGF-β诱导胶原 、胶原 大量产生和堆积 ,引起静脉移植物内膜增生和狭窄 ;阻断 ANG 的作用可明显减轻静脉移植物内膜增生。Objective To find an effective way for preventing intimal hyperplasia of venous grafts, the effect of transforming growth factor-β(TGF-β)and angiotensin Ⅱ(ANGⅡ) on intimal hyperplasia of venous grafts and the relationship between them were studied. Methods Twenty New Zealand rabbits were randomly divided into two groups: treatment group and control group. Each group contains 10 rabbits. External jugular veins of New Zealand rabbits were harvested and grafted into the ipsilateral carotid artery in both groups. After operation, in addition to common chow, rabbits in treatment group were given ANG Ⅱ type 1 receptor blocker (Losartan) 25mg·kg -1/d by oral administration until sacrificed, meanwhile rabbits in control group were fed with placebo. The rabbits were sacrificed at 4 weeks after operation, and with the semiquantitative method mRNA levels of TGF-β, collagen Ⅰ and collagen Ⅲ in venous grafts were measured. Results There were a remarkable decrease in mRNA levels of TGF-β, collagen Ⅰ, collagen Ⅲ and the ratios of values of picture element in treatment group versus control group (P<0.01). Conclusions TGF-β and ANG Ⅱhave an important role in intimal hyperplasia of venous grafts.ANG Ⅱ's binding to ANG Ⅱ type 1 receptor upregulates the levels of TGF-β, and TGF-β induces the production of collagen I and collagen Ⅲ directly which give rise to the intimal hyperplasia of venous grafts. Blocking the effect of ANG Ⅱ can obviously reduce the intimal hyperplasia.

关 键 词：转化生长因子Β 静脉移植物 内膜增生 血管紧张素Ⅱ 大白兔 心血管外科 动物模型 

分 类 号：R654.3[医药卫生—外科学]