用随机扩增多态性DNA(RAPD)技术区分不同地理株白纹伊蚊  

Genetic Differentiation of Aedes albopictus Strains Detected By the Random Amplified Polymorphism DNA Polymerase Chain Reaction (RAPD-PCR)

作　　者：李春晓[1] 朱礼华[1] 董言德[1] 赵彤言[1] 陆宝麟 

机构地区：[1]军事医学科学院微生物流行病研究所全军媒介生物学重点实验室,北京100071

出　　处：《中国媒介生物学及控制杂志》2003年第4期248-250,共3页Chinese Journal of Vector Biology and Control

基　　金：国家自然基金项目资助 (39970 665)

摘　　要：目的　对来自北京、广州、成都、上海和日本的冲绳及长乐寺 6个不同地理株的 12只雄蚊进行随机扩增多态性DNA(RAPD)分析。方法　RAPD -PCR。结果　UPGMA法构建的系统聚类图表明 ,12只白纹伊蚊可归为明显不同的两个组 ,不同地理株之间存在着显著的遗传分化。结论　用RAPD法可以区分不同地理株的白纹伊蚊。Objective To estimate gene relationships and identification of 6 Aedes albopictus geographical populations from Beijing, Guangzhou, Shanghai, Chengdu, Okinawa and Nagarakuzi. Methods RAPD-PCR. Results Molecular phylogenetic tree derived from genetic distance suggested that the RAPDs are most related to geographic conditions. The Aedes albopictus populations were separated into the two major branches of dendrograms. There were genetic divergences among different geographical population. Conclusion RAPD-PCR technology can be used for identification of different Aedes albopictus geographical strain.

关 键 词：随机扩增多态性DNA RAPD 地理株 白纹伊蚊 媒介 

分 类 号：R384.1[医药卫生—医学寄生虫学]