香蕉离体试管芽诱变育种的研究 Ⅴ.漳蕉8号株系基因组变异检测  

MUTATION BREEDING OF BANANA TUBE-BUDS IN VITRO Ⅴ. GENOMIC VARIATION OF ZHANGJIAO No.8 STRAIN DETECTED BY RAPD

作　　者：郭建辉[1] 沈明山[2] 蔡恩兴[1] 洪富祥[2] 陈丽萍[1] 黄锡栋[1] 

机构地区：[1]福建省漳州市农业局,福建漳州363000 [2]厦门大学生命科学学院,福建厦门361005

出　　处：《核农学报》2003年第4期255-258,共4页Journal of Nuclear Agricultural Sciences

摘　　要：应用 2 0条Sangon的随机引物 ,对6 0 Coγ射线辐照诱变所获新株系漳蕉 8号(原漳农 8号 )及其对照品种台湾北蕉 (MusAAAGrandCavendish)的总DNA进行RAPD ,结果发现有 1 2条引物扩增出 68条谱带 ,其中同源带 53条 ,占总带数的77 9% ,差异带 1 5条 ,占总带数的 2 2 1 % ;漳蕉 8号与对照多态性差异高达 2 4 6% ,且显示出稳定性遗传。其中S10 和S19的 3条差异带 ,可作为漳蕉 8号株系鉴定和防退化复壮的筛选标记。Zhangjiao No.8,a new variety induced from irradiation by 60 Co γ rays,was analyzed by RAPD using 20 random primers against Mus AAA Grand Cavendish. The result showed that 68 bands could be amplified by 12 primers,53 were homologous,accounted for 77 9% of total bands; only 22 1% were differential bands,the polymorphism difference up to 24 6% between Zhangjiao No.8 and CK,indicating its stable heredity.Three differential bands from S 10 and S 19 could be used as markers for identification and anti degeneration of Zhangjiao No.8.

关 键 词：香蕉 离体试管芽 诱变育种 漳蕉8号株系 基因组变异 检测 辐照诱变 RAPD 

分 类 号：S668.1[农业科学—果树学]