铈氨基酸配合物对Oliogo DNA的切断  

Study for the cleavage reaction of oligodeoxyribonucleotide by cerium ions and amino acid complexes

作　　者：蒯丽衡[1] 沈鹤柏[1] 冯旦[1] 周文骏[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《化学研究与应用》2003年第4期530-532,共3页Chemical Research and Application

基　　金：上海市自然科学基金(002A14042);上海市科学技术委员会资助项目(011511021)

摘　　要：Cyclic voltammetry(CV)and ultravioletvisible spectroscopy(UV) were used to study cerium amino acid complexes.The amino acids are Phenylananine,Histidine,Serine,Threonine and Glutamicacid.These amino acids can coordinate with cerium ion(Ⅳ) to form complexes at near neutral condition(pH=7).At the same time,the denaturing ployacrylamidegel electrophoresissilver dye method was used to analyze the hydrolysis of 26mers oligodeoxyribonucleotide by cerium amino acid complexes around the neutral condition(pH=7).The results show that these cerium amino acid complexes can cleavage DNA at different degree.We also analyze the possible mechanism of the different hydrolysis effects.Cyclic voltammetry(CV)and ultravioletvisible spectroscopy(UV) were used to study cerium amino acid complexes.The amino acids are Phenylananine,Histidine,Serine,Threonine and Glutamicacid.These amino acids can coordinate with cerium ion(Ⅳ) to form complexes at near neutral condition(pH=7).At the same time,the denaturing ployacrylamidegel electrophoresissilver dye method was used to analyze the hydrolysis of 26mers oligodeoxyribonucleotide by cerium amino acid complexes around the neutral condition(pH=7).The results show that these cerium amino acid complexes can cleavage DNA at different degree.We also analyze the possible mechanism of the different hydrolysis effects.

关 键 词：铈氨基酸配合物 OliogoDNA 切断DNA 差示UV CU 电泳 单链寡聚脱氧核苷酸 

分 类 号：Q523[生物学—生物化学] Q503