AFLP和RAPD标记技术在栉孔扇贝遗传多样性研究中的应用比较  

Comparison of Genetic Diversity between Populations of Chlamys farreri Revealed by AFLP and RAPD Analysis

作　　者：王玲玲[1,2] 宋林生[1] 李红蕾[1] 胥炜[1] 李俊强[1] 常亚青[3] 

机构地区：[1]中国科学院海洋研究所 [2]中国科学院研究生院北京100039 [3]大连水产学院

出　　处：《动物学杂志》2003年第4期35-39,共5页Chinese Journal of Zoology

基　　金：国家重点基础研究发展规划项目资助 (No.G19990 12 0 0 8)

摘　　要：AFLP和RAPD标记技术是近年来发展最快的基于PCR基础上的两种DNA标记技术 ,本文比较了两种标记技术在我国栉孔扇贝群体遗传多样性研究中的应用。共筛选 2 0个RAPD引物和 7个AFLP引物组合 ,检测到AFLP标记的有效等位基因数和平均多态信息量稍低于RAPD标记 ,但AFLP标记在每单位分析中扩增到的野生和养殖群体的多态性条带数 ( 2 3 8,2 4 8)分别高于RAPD标记 ( 5 6,5 6) ,AFLP多态性检测效率显著高于RAPD标记。AFLP和RAPD两种标记技术所揭示的野生种群与养殖群体间的近交系数、遗传距离两项指标均表明 ,我国栉孔扇贝养殖群体和野生种群之间尚未出现明显的遗传分化。研究结果表明 :RAPD和AFLP这两种标记技术均可用于栉孔扇贝遗传多样性的分析 ,其分析结果是一致的。AFLP (Amplified Fragment Length Polymorphism) and RAPD (Randomly Amplified Polymorphism DNA) techniques were used to study the genetic diversity of Chlamys farreri. The AFLP marker system has lower polymorphism information content (PIC) and lower effective numbers of alleles per locus (Ne) than RAPD but it has a much higher assay efficiency index (Ai) detected by 7 AFLP primer combinations and 20 RAPD primers. The inbreeding index was 0 038?6 and 0 028?3 and genetic distance was 0 028?0 and 0 027?1 as revealed by AFLP and RAPD respectively. This result indicates that there is no genetic differentiation between the natural population and hatchery stock and both AFLP and RAPD techniques are useful for the analysis of the genetic diversity of Zhikong scallop populations.

关 键 词：栉孔扇贝 AFLP RAPD 遗传多样性 群体 

分 类 号：Q953[生物学—动物学]