超级杂交稻两优培九及其亲本的光氧化特性  

Photooxidation characteristics of super-hybrid rice "Liangyoupeijiu" and its parents

作　　者：王荣富[1] 张云华[1] 钱立生[1] 于江龙[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036

出　　处：《应用生态学报》2003年第8期1309-1312,共4页Chinese Journal of Applied Ecology

基　　金：国家重点基础研究发展规划项目(G19980 10 10 0 );安徽省自然科学基金资助项目( 0 10 4 110 6 )

摘　　要：通过比较超级杂交稻两优培九和其亲本的光能利用与活性氧代谢的差异 ,为培育耐光氧化的杂交稻提供选亲配组的生理依据 .使用TPS 光合仪和FMS2荧光仪 (Hansatech ,UK)分别测定了人工光氧化处理后水稻叶片的光合速率与叶绿素荧光 ,同时测定了叶绿素、蛋白质和丙二醛的含量 ,以及超氧化物歧化酶和过氧化物酶的活性 .结果表明 ,两优培九的光合速率低于母本 2 .4% ,高于父本 2 3 % ;光氧化处理 8d后 ,与母本相比 ,两优培九的叶绿素、蛋白质、原初光化学效率、超氧化物歧化酶和过氧化物酶分别高于母本 33 %、1 5 %、30 %、32 %和 1 0 0 % ;光化学猝灭系数和丙二醛分别低于母本 9%和 50 % ,与父本相比 ,则差异不大 .超级杂交稻两优培九在光氧化条件下光能利用率较高 。With comparison of the differences of light absorption and active oxygen metabolism between super hybrid rice 'Liangyoupeijiu' and its parents, this paper provided a physiological foundation of matching and grouping for cultivating anti photooxidized hybrid rice. TPS photosynthetic system and FMS2 fluorescence (Hansatech, UK) were used to respectively determine the photosynthetic rate and the chlorophyll fluorescence in the rice leaves that had been processed in artificial photooxidization. Meanwhile, the contents of chlorophyll, protein and malonaldehydic acid (MDA) as well as the activation of superoxide dismutase (SOD) and peroxidase (POD) were determined. The results showed that the photosynthetic rate of 'Liangyoupeijiu' was 2.4% lower than that of its female parent, and 23% higher than that of its male parent. After eight days anti photooxidization process, its chlorophyll content was higher than that of its female parent by 33%, protein by 15%, initial photochemical efficiency by 30%, SOD by 32% and POD by 100%, while its photochemical quince coefficient was lower than that of its female parent by 9% and MDA by 50%. It showed that there was not much difference between the filial generation and its male parent. The super hybrid rice 'Liangyoupeijiu' was very efficient for solar energy utilization under the condition of photooxidization, and had outgrown its parents in the aspect of anti photooxidization.

关 键 词：超级杂交稻 光氧化 叶绿素荧光特性 膜脂过氧化 

分 类 号：S511[农业科学—作物学]