EB病毒抗原单克隆抗体的特异性研究  

Specific Study of Monoclonal Antibodies to Epstein-Barrvirus

作　　者：李士瑛[1] Roel A.de Weger Henk-Jan Schuurman 

机构地区：[1]山西医学院病理教研室,太原030001 [2]荷兰乌特勒支大学病理系

出　　处：《临床与实验病理学杂志》1992年第3期195-197,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：5种EB病毒细胞系通过三步放大间接免疫过氧化物酶方法,检测抗EB病毒抗原的单克隆抗体(McAb)—病毒壳抗原(VCA)、膜抗原(MA),早期抗原(EA)、早期弥漫性抗原(EA—D)、早期限制性抗原(EA—R)、核抗原(EBNA)、核抗原第2型(EBNA—2)及潜在性膜蛋白(LMP)。结果表明McAb、EBNA、EBNA—2和EA有较高特异性,VCA和EA—R有一定特异性,MA、LMP和EA—D未得到预期的特异性反应。Several monoclonal antibodies(McAbs) to Epstein—Barrvirus(EB) antigen—Viral capsid antigen (VGA), membrane antigen(MA), early antigen(EA), early antigen—diffuse (EA—D), early antigen—restricted (EA—R), EB unclear antigen (EBNA), EBNA—2 and latent membrane protein (LMP) were tested using a 3 steps amplified indirect immunoperoxidase method on 5 EB cell lines. The results indicate a high specificity of McAbs, EBNA, EBNA—2 and EA. VCA and EA—R show specificity to a certain extent. MA, LMP and EA—D do not present expected specific reaction.

关 键 词：EB病毒抗原 细胞系 免疫组织化学 

分 类 号：R392.3[医药卫生—免疫学]