中国人内源性高甘油三酯血症中ApoB基因第26外显子XbaI多态性分析  

Analysis of XbaI polymorphism in the ApoB gene to hypertriglyceridemics in Chinese population

作　　者：宫立国[1] 刘晓茹[1] 邱广斌[1] 李海峰[1] 崔娴维[1] 

机构地区：[1]沈阳军区医学检验中心,辽宁沈阳110003

出　　处：《中国实验诊断学》2003年第4期306-308,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　对中国人内源性高甘油三酯血症 (hypertriglyceridemics ,HTG)中ApoB基因第 2 6外显子XbaI多态性进行分析。方法　采用聚合酶链反应和限制性片段长度多态性 (PCR RFLP)方法 ,对沈阳地区 115名HTG患者及 15 0名血脂正常健康成人ApoB基因第 2 6外显子XbaI多态性进行分析 ;同时测定血脂及载脂蛋白水平 ,分析ApoB基因XbaI多态性对血脂及载脂蛋白水平的影响。结果　正常对照组及HTG组中基因型均以X -X -为主。HTG组X +等位基因分布频率及X +X -基因型分布频率均显著高于对照组 (13.5 %vs 4 .0 % ,P <0 .0 1;2 5 .2 %vs 8.0 % ,P <0 .0 1)。HTG组中 ,X +X -基因型者TG、TC、LDL C、ApoB水平均高于X -X -基因型者 (TG、ApoB水平P <0 .0 5 ;TC、LDL C水平P <0 .0 1)。结论　ApoB基因第 2 6外显子XbaI多态性与中国人内源性高甘油三酯血症的遗传易感性有一定关联 ,X +等位基因的存在可能是中国人HTG发生的一个重要因素。Objective To analyze XbaI polymorphism of ApoB gene(on the 26 exon) in Chinese population with hypertriglyceridemics. Methods XbaI restriction fragment length polymorphism in the ApoB gene on samples of 115 HTG patients and 150 age-matched healthy individuals in Shenyang area were detected with the method of PCR-RFLP;the effect of this polymorphism on the level of blood lipids was analyzed.Results Most genotype in HTG groups and control groups is X-X-.The X+ allelic frequency and X+X- genotype frequency of ApoB gene for HTG cases are higher than those for control groups(13.5% vs 4.0%, P <0.01; 25.2% vs 8.0%, P <0.01).The serum levels of TG、TC、LDL-C、ApoB in X+X- genotype are higher than those in X-X- genotype(TG、ApoB: P < 0.05 ;TC,LDL-C: P <0.01). Conclusion The results suggest that XbaI polymorphism of ApoB gene is associated with susceptibility to hypertriglyceridemics in Chinese population.The existence of X+ allelic may be an important agent leading to hypertriglyceridemics in Chinese population.

关 键 词：中国人 内源性高甘油三酯血症 APOB基因 26外显子 XBAI 多态性 分析 

分 类 号：R589.2[医药卫生—内分泌]