石蜡包埋组织中提取DNA应用于PCR反应影响因素的研究  

Factors that influence PCR of the DNA extraction from paraffin-embedded tissues

作　　者：赵吉生[1] 盖宝东[1] 森隆弘[2] 滕盛启成[2] 大内宪明[2] 里见进[2] 张德恒[1] 

机构地区：[1]吉林大学中日联谊医院基本外科,吉林长春130031 [2]日本东北大学医学部

出　　处：《中国实验诊断学》2003年第4期330-331,共2页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　探讨石蜡包埋组织中提取DNA应用于PCR反应的影响因素。方法　应用从石蜡包埋组织中提取的DNA ,PCR反应扩增甲状腺髓样癌RET基因第 11外显子 ,分析石蜡包埋组织中提取DNA应用于PCR反应的影响因素。结果　 10 .0 μm× 1组织切片组 ,PCR反应循环次数 4 0次组 ,PCR反应模板量 0 .0 5 μg组PCR反应取得高质量目的基因DNA。结论　减少提取组织用量有利于减少抑制PCR物质的量 ,有利于反应的成功 ;PCR反应循环次数一般应大于 4 0次 ;PCR反应时减少模板量有利于PCR反应成功。Objective Factors influenced PCR of the DNA extraction from paraffin- embedded tissues were investigated.Methods Genomic DNA was extracted from one case of paraffin-embadded sporadic medullary thyroid carcinoma samples, exon 11 of RET gene was amplified by polymerase chain reaction, different factors influenced PCR were analyzed.Results The condition of 10.0 μm×1 slice group, 40 cycles, 0.05 μg template get high quality aim DNA.Conclusion Reduce the amount of paraffin-embedded tissues used for DNA extraction and reduce the amount of PCR template are good for obtaining high quality aim DNA, PCR cycles times should be more than 40.

关 键 词：石蜡包埋组织 DNA PCR反应 影响因素 研究 

分 类 号：R446.8[医药卫生—诊断学]