高效表达人IL-2工程菌株的稳定性监测  

Monitor for Stability of High Expression IL-2 Strain

作　　者：关晓峰[1] 郭桥[1] 徐福平[1] 魏宪志[1] 宋昌玲[1] 冷梅[1] 

机构地区：[1]长春生物制品研究所,吉林长春130062

出　　处：《深圳中西医结合杂志》2003年第4期264-266,共3页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的　为IL - 2的规模化生产提供可靠的生产用菌种。方法　冻干 2年的工作种子批经理化、微生物、生物学活性等分子水平的检测 ,进行定期监测。结果　经全面检测 ,包括基因序列分析等与原始菌种一致。结论　高效重组人IL - 2生产用菌种 ,符合《中国生物制品规程》2 0 0 0版检标准 ,冻干菌种 - 2 0℃保存 2年 。Objective To supply reliable bacteria strain for the large-scale production of IL-2.Methods 2 years lyophilized working strain stock was assayed for its physical,chemical,microbiological and biological characteristics at intervals.Results All the characteristics including gene sequences are identical to the original strain.Conclusion The recombinant IL-2 strain is complied with the standards of 'Regulation of Biological Products of China',2000 press.Lyophilized stock strain frozen at-20℃ for 2 years is pretty stable.

关 键 词：工程菌 IL-2质粒 表达 

分 类 号：Q93-33[生物学—微生物学]