小鼠胎肝基质细胞体外扩增骨髓来源的LTC-IC  

Expansion of Bone Marrow LTC-ICs in vitro by Mouse Fetal Liver-derived Stromal Cell Lines

作　　者：苑春慧[1] 刘兵[1] 吴英[1] 张毅[1] 毛宁[1] 

机构地区：[1]军事医学科学院基础医学研究所细胞生物学研究室,北京100850

出　　处：《生物工程学报》2003年第4期450-455,共6页Chinese Journal of Biotechnology

基　　金：国家高技术研究发展计划"863"资助项目 (No.2 0 0 1AA2 17131)~~

摘　　要：基质细胞是胎肝造血微环境的主要成分 ,参与造血干 祖细胞的自我更新、增殖分化的调控。为了研究小鼠胎肝基质细胞在造血微环境中的功能 ,采用转染SV40大T抗原基因的方法建立了小鼠胚胎期第 12 5天 (Embry onicday 12 5 ,E12 5d)胎肝基质细胞系A4、B3 ,并进一步鉴定基质细胞系的一般细胞生物学特性和造血支持功能。结果 :A4、B3为细胞形态、生长行为以及表面分子表达不同细胞系 ,二者均可维持骨髓源长期培养启动细胞 (Long termculture initiatingcell,LTC IC)至少 4周并且有不同程度的扩增LTC IC能力 ,其中B3扩增LTC IC的能力是A4的8 3倍。外源性细胞因子组合SCF +IL 3 +IL 6+Epo在本实验体系中不影响LTC IC数量的维持和扩增。暗示E12 5d胎肝造血微环境中基质细胞的功能是不同的 。As main component of fetal liver hematopoietic microenvironment, different stromal cells may play distinct roles in the regulation of hematopoietic stem cell self-renewal, proliferation and differentiation. It is a unique approach to establish stromal cell lines for analyzing the interaction of hematopoietic cells with the stroma on the clonal level to dissect the function of hematopoietic microenvironment. In this study two immortal stromal cell lines-A4, B3 were established from mouse embryonic day 12 5 fetal liver by transfection of pSV 3neo plasmid. A4 exhibited a fibroblast-like morphology, 25 hours population doubling time as well as high levels of CD29, CD44, UEA-1 and low levels of CD105 expression. In contrast B3 displayed an epithelium-like morphology, 37 hours population doubling time along with high levels of CD105 and low levels of UEA-1 expression. In addition no or low levels of CD31, CD34, CD45 and CD144 expression were found in the two cell lines. These results indicate that A4, B3 are two discriminating cell lines in terms of morphological characters, growth behaviors and surface molecular expression types. Next functional assays using Limited-Diluted Assay(LDA) and Bulk-LTC-IC were done: both stromal cell lines had similar ability to maintain the survival proportion of inoculated mouse bone marrow-derived Long-Term Culture-Initiating Cells(LTC-ICs), and they could also support LTC-ICs expansion up to 4 weeks by co-culture in vitro. More strikingly, B3 could expand the absolute number of LTC-ICs over 13-fold at week 4 than that of week 0, and the ability of B3 to expand absolute number of LTC-ICs was over 8-fold of that of A4. Proportions of LTC-ICs in proliferating cell populations in two long-term culture systems was similar at week 4, no matter with or without extra cytokines. Further study indicated that the ability of LTC-ICs to yield CFCs was held as the number 6(±1 2) after 4 weeks co-culture. Extra cytokines-SCF+IL-3+IL-6+Epo had no influence in the maintenance and expansion of LTC

关 键 词：小鼠 胎肝基质细胞 体外扩增 骨髓 LTC-IC 造血器官 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]