成人骨髓间充质干细胞体外培养扩增及其生物学特性研究  

The in vitro expansion and biological characteristics of adult human bone marrow-derived mesenchymal stem cells

作　　者：罗依[1] 梁彬[1] 余勤[2] 

机构地区：[1]浙江大学医学院附属第一医院,杭州310003 [2]浙江中医学院

出　　处：《浙江医学》2003年第8期468-469,473,共3页Zhejiang Medical Journal

基　　金：浙江省科技厅重点资助项目 (2003c23015)

摘　　要：目的 建立成人骨髓间充质干细胞 (MSCs)体外培养和扩增的条件 ,探讨其生物学特性。方法 取正常成人骨髓用含10%胎牛血清的LG -DMEM培养液培养、扩增后 ,进行透射电镜观察 ,流式细胞仪行细胞表面抗原及细胞周期分析检测。结果 培养扩增获取的成人骨髓MSCs形态均一 ,增殖能力强 ,细胞扩增至第5代细胞数达 (2～3)×108。该类细胞CD29、CD44、CD166表达阳性 ,CD14、CD34、CD45、HLA -DR表达阴性。细胞周期以G0/G1 期为主。细胞胞浆具丰富粗面内质网。结论 所建立的分离和培养方法可获取骨髓粘附细胞中一组独特的细胞群 ,具有MSCs的生物学特性。Objective To establish the method of in vitro culture and expansion of adult human bone marrow-derived mesenchymal stem cells (MSCs) and to investigate their biological characteristics.Methods Adult human MSCs cultured in Dulbecco's Modified Eagle's Medium with low glucose containing 10% fetal bovine serum. The FACS was performed to examine the expression of cell surface molecules and DNA content. Results The MSCs yielded in this study were homogenous population and had multiplication potential. Over (2～3)×10 8 MSCs would be obtained from each 10-ml bone marrow aspirate at 5th passage. The cells were positive for CD29、CD44、CD166, and negative for CD14、CD34、CD45、HLA-DR.Conclusion The method can isolate and culture a homogenous MSC population that have unique growth phenotype.

关 键 词：骨髓间充质干细胞 体外培养 扩增 生物学特性 研究 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]