一株引起普通棉耳狨猴死亡病毒的分离与鉴定  

Isolation and Identification of a Lethal Viral Pathogen From Common Cotton-eared Marmosets

作　　者：石建党[1] 李晓眠[1] 刘凤勇[2] 金孟珏[2] 张国际[1] 刘民[1] 李梅[1] 

机构地区：[1]天津医科大学微生物教研室,天津300070 [2]天津医科大学动物中心,天津300070

出　　处：《中国病毒学》2003年第4期357-361,共5页Virologica Sinica

基　　金：天津市教委基金(01-20814)

摘　　要：1999年6月,天津医科大学动物中心饲养的珍贵灵长类实验动物——普通棉耳狨猴群体中暴发急性呼吸道传染病,病死率高达33％。在排除细菌感染的基础上,通过死亡狨猴肺组织匀浆接种鸡胚和MDCK细胞的分离培养,分离出一株具有高血凝效价的病毒株。经双份血清试验及动物接种试验,确认该病毒是本次疾病流行的病原体。又进一步通过与常见呼吸道病毒标准毒株及血清进行交叉血凝抑制试验、电镜观察、RT-PCR技术并结合生物信息学方法对该毒株进行鉴定,确认本次疾病流行的病原体是副流感1型病毒中的仙台病毒。As the close consanguinity of human race, the common cotton-eared marmoset (Callithrix Jacchus) has been used in a wide range of research as a primate model. Acute respiratory infection (ARI) broke out in a colony reared in the experimental animal center of Tianjin Medical University in 1999. A virus strain with high hemagglutinin titer was isolated out from embryonated eggs and MDCK cells after inoculating with the lung tissues suspension of the dead marmosets. The data of the hemagglutination inhibition (HI) test with paried serum samples and the animal inoculation test confirmed that the isolated virus strain is the pathogen of the ARI. The isolated virus strain was then identified by the cross HI test, electron microscope observation, RT-PCR and BLAST analyses. The virus was verified to be Sendai virus, which belongs to Parainfluenza virus type 1.

关 键 词：棉耳狨猴 病毒 分离 鉴定 血凝抑制试验 仙台病毒 

分 类 号：S852.65[农业科学—基础兽医学]