一种浓缩噬藻体的反冲超滤技术  

A Backflushing Ultrafiltration Technique for Concentrating Cyanophage

作　　者：罗文清[1] 鞠川 程凯[1] 赵以军[1] 石正丽[2] 

机构地区：[1]华中师范大学生命科学学院,湖北武汉430079 [2]中国科学院武汉病毒研究所无脊椎动物病毒学联合开放实验室,湖北武汉430071

出　　处：《中国病毒学》2003年第4期397-400,共4页Virologica Sinica

基　　金：国家自然科学基金(3960004;399700647);中国科学院院长基金(院基计字892号)

摘　　要：噬藻体(Cyanophage)是一类感染蓝藻的病毒,形态上同于噬菌体[1],近期的研究表明,噬藻体作为水体环境中活跃的动态因子,在控制水体初级生产力和有害藻类水华(Harmful Algal Bloom,HAB)方面可能发挥着重要的作用[2,3],甚至影响水体生态系统中食物链的结构[4],因此研究水体中噬藻体的生理生态学特性对于了解其生态功能是非常重要的,但是由于自然水体中的噬藻体浓度往往较低,难以直接对其进行定性或定量研究,所以对天然水样中的噬藻体进行高效、快速的浓缩是研究噬藻体生态地位和功能的关键和难点.Most of the cyanophages were adsorbed onto the ultrafilter membrance when pre-filtrated water sample passed through the tangential flow ultrafiltration system. In order to recover much more cyanophages, we used a technique named 'backflushing ultrafiltration'. The results indicated that when the volume of the backflush liquid was raised step by step, the concentration multiple of the cyanophage would gradually decrease, while the recovery rate of the cyanophage would gradually increase. In our performance, the maximal concentration multiple and the recovery rate of cyanophage could reach up to 30 times and more than 90% severally. This method was tested to be effective for concentrating cyanophage from large volume liquid sample. Moreover, the concertration multiple of the cyanophage could be elevated to nearly one-hundred times when two-step backflushing ultrafiltration was adopted.

关 键 词：噬藻体 反冲超滤技术 浓缩 蓝藻 病毒 

分 类 号：Q93-3[生物学—微生物学]