卡铂的反相高效液相色谱法测定  

THE DETERMINATION OF CARBOPLATIN BY REVERSAL REVERSAL PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：魏培海[1] 张延昌[1] 许青[1] 

机构地区：[1]山东教育学院生物化学系,济南250013

出　　处：《山东师范大学学报（自然科学版）》2003年第3期36-38,共3页Journal of Shandong Normal University(Natural Science)

摘　　要：建立了卡铂的反相高效液相色谱测定方法 .以甲醇 -水 ( 30 :70V V)为流动相 ,流速为 1.2ml·min- 1 ,卡铂与杂质完全分离 ,检测波长为 2 38nm时 ,测量的线性范围为 4～ 48μg·ml- 1 ,平均回收率大于 99% .此法简单、快速 。The determination of carboplatin by high performance liquid chromatography was reported. Using methanol-water (30:70 V/V)as mobile phase and controlling the flow rate at 1.2 ml·min -1, carboplatin was completely separated from the impurity.The linear range is from 4 to 48 μg·ml -1 at the detection wavelength of 238 nm,and the average recoveries of carboplatin are 99%.The method is simple and sensitive,which would be suitable for the determination of both carboplatin and carboplatin preparation.

关 键 词：卡铂 反相高效液相色谱法 含量测定 流动相 铂类抗肿瘤药物 质量控制 

分 类 号：R979.1[医药卫生—药品] R927.2[医药卫生—药学]