水稻雄性不育恢复系明恢63的感光基因分析  

Analysis of Photoperiod-sensitivity Genes in Minghui63,an Restorer Line of indica Rice( Oryza sativa L.)

作　　者：罗林广[1] 徐俊峰[1] 翟虎渠[1] 万建民[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室

出　　处：《Acta Genetica Sinica》2003年第9期804-810,共7页

基　　金：教育部优秀骨干教师基金;农业部超级稻研究计划 (Q2 0 0 0 2 5 )~~

摘　　要：水稻恢复系明恢 6 3是中国应用面积最大、利用最广泛的恢复系。利用抽穗期感光性近等基因系EG0～EG7及ER～LR对明恢 6 3进行的分析表明 ,明恢 6 3在E1 、E2 、E3位点分别带有E1 、e2 、E3基因 ,在Se 1位点带无感光功能的Se 1e 基因。进一步用抽穗期QTL近等基因系NIL(Hd1)和NIL(Hd4 )进行的研究表明 ,明恢 6 3带有显性感光基因E1 和无感光功能的Se 1e 基因 ,并推测明恢 6 3带有能抑制E1 基因表达的隐性抑制基因。认为籼型杂交稻抽穗期受不育系和恢复系感光基因及感光性抑制基因的共同作用。初步讨论了明恢 6 3广适性的遗传基础。Hybrid rice is very important in agriculture production in China.Its selecting property makes it significant to study the genetic performance of F 1's date to heading (DH).Minghui63,an indica rice restorer line,has been wildly applied to hybrid rice seed production in China,but the photoperiod sensitivity gene of heading date in this restorer line is still unknown.This definitely limited the further use of this restorer line in breeding practice and re production of hybrid seeds.To solve this problem,using heading time nearly isogenic lines EG0～EG7?ER LR and two heading date QTL isogenic lines,NIL ( Hd1 ) and NIL ( Hd4 ),with the genes of Nipponbare but Hd1 (Se 1 ) and Hd4 (E 1 ) genes from Kasalath,respectively,we performed a genetic analysis of Minghui63 with special reference to photoperiod sensitivity loci,using natural long days in Nanjing(32°N) and natural short days in Linshui county,Hainan province(18°29′N),where the average day length is about 14 h and 11 6 h during the course of rice growing,respectively.The F 1 and F 2 generations from the crosses 'heading time nearly isogenic lines × Minghui63' were subjected to genetic analyses.Experimental results showed that Minghui63 carries photoperiod sensitivity allele gene E 1 and E 3 in E 1 and E 3 loci,respectly,and a photoperiod insensitivity allele Se 1 e in Se 1 locus,and it also carries a recessive inhibitor for photoperiod sensitivity gene E 1 .Meanwhile,the photoperiod sensitive genes, E 1 and the photoperiod insensitive genes, Se 1 e ,in Minghui63 were also identified by crossing with the nearly isogenic lines for heading time QTLs ,NIL( Hd1 )and NIL( Hd4 ).The results indicated that Minghui63's genotype of heading date was: E 1E 1e 2e 2E 3E 3Se 1 eSe 1 e . The result from this research indicated that Minghui63 carries a major dominant photoperiod sensitive gene E 1 in E 1 locus,and our previous researches indicated that Zhenshan97A carried a ma

关 键 词：水稻(Oryza SATIVA L.) 近等基因系 抽穗期 感光基因 

分 类 号：S511[农业科学—作物学]