猪a1-岩藻糖转移酶基因(FUT1)在26个中外猪种中的遗传变异研究(英文)  

Research on the Genetic Variations of a1-Fucosytransferase ( FUT1 ) Gene in 26 Pig Breeds

作　　者：晏学明[1] 任军[1] 郭源梅[1] 丁能水[1] 陈克飞[1] 高军[1] 艾华水[1] 陈从英[1] 麻骏武[1] 黄路生[1] 

机构地区：[1]江西农业大学江西省动物生物技术重点开放实验室,南昌330045

出　　处：《Acta Genetica Sinica》2003年第9期830-834,共5页

基　　金：国家自然科学基金 (编号 :3 0 0 60 0 5 8)项目资助~~

摘　　要：肠毒素大肠杆菌F18(ECF18)是引起仔猪断奶后水肿和腹泻病的主要病原菌 ,a1 岩藻糖转移酶基因(FUT1)是ECF18侵染猪小肠的受体蛋白候选基因。通过采用PCR RFLP方法检测了 5个西方商业猪种以及 2 1个中国地方猪种 (群 ) 14 5 8个个体在FUT1基因开放阅读框架的 30 7核苷酸位点的G A点突变 (M30 7G A)遗传变异。结果表明 :5个外来猪种以及中国地方猪种中的临高猪在该FUT1基因位点存在多态性 ,其他中国地方猪种均表现为极端的单态分布 ,只有易感的GG基因型 ,没有多态性。由此提示 :1)如果猪FUT1M30 7G A 点突变是决定猪小肠上ECF18受体表达与否的关键因素 ,则绝大部分中国地方猪种均不具备抵抗ECF18的遗传基础 ,这除了表明ECF18抗性基因有可能起源于西方猪种外 ,同时也表明对中国地方猪种中在这个位点惟一存在多态性的海南临高猪的品种资源保存具有非常重要的意义。 2 )一般而言 ,在中国的养猪生产实践中 ,中国地方猪种的仔猪抗水肿与腹泻病能力普遍强于外来猪种 ,研究的结果提示有必要对中国地方猪种所具备的上述遗传抗性做更深入的研究 ,寻找、定位其相应的QTL或 和抗性基因。Enterotoxigenic Escherichia coli F18(ECF18) is a main pathogen that causes edema disease and post weaning diarrhoea in piglets,and a1 fucosytransferase( FUT1 ) gene has been identified as a candidate gene for controlling the expression of the receptor for ECF18 bacteria.The genetic variations at position 307 nucleotide in open reading frame of FUT1 gene in 26 pig breeds (total 1458 individuals) from 5 western commercial pig breeds and 21 Chinese native pig breeds were investigated by PCR RFLP.The results showed that the genetic polymorphisms of the FUT1 locus were only detected in 5 western pig breeds and the Chinese Lingao pig breed,5 western pig breeds possessed 3 different genotypes,and Lingao pig breed had two susceptible genotypes GG and AG ,while all the other 20 Chinese native pig breeds only presented the susceptible genotype GG .The results indicated that if M307 G A point mutation in the coding region of FUT1 gene was the key factor determining the expression of the ECF18 receptor,most of Chinese native pig breeds were absent of the genetic background on the resistance to ECF18 bacteria.In this case,it was inferred that the resistance gene to ECF18 might be originated from western pig breeds.In addition,it is of great importance for the conservation of Lingao pig breed as it is the only found Chinese native pig breed possessing resistance M307 A allele in FUT1 gene.Generally,compared with exotic pig breeds,Chinese native pig breeds have stronger resistance to edema disease and post weaning diarrhoea in piglets.The results suggested that further study should be done to identify and characterize putative QTL (quantitative trait locus) or/and the functional gene responsible for the resistance to ECF18 in Chinese native pig breeds.

关 键 词：猪 大肠杆菌F18 FUT1基因 遗传变异 

分 类 号：S852.6[农业科学—基础兽医学]