胶体金纳米粒子及其蛋白标记物在电场中的泳动行为研究  

Electrophoresis of bare and proteins-capped gold colloidal nanoparticles

作　　者：虞伟[1] 廖建辉[1] 葛存旺[1] 顾宁[1] 武建国[2] 

机构地区：[1]东南大学教育部分子与生物分子电子学开放实验室,江苏南京210096 [2]南京军区南京总医院全军医学检验中心临床免疫实验室,江苏南京210002

出　　处：《微纳电子技术》2003年第9期35-38,共4页Micronanoelectronic Technology

基　　金：国家自然科学基金资助项目(60171005);教育部优秀青年教师科研教学奖励基金资助项目(2000年)

摘　　要：采用柠檬酸钠还原法制备了3种不同粒径的胶体金纳米粒子,分别用于与蛋白质分子标记。同时以琼脂糖凝胶为支持介质,观察其在电场条件下的泳动行为特征,并进行对比研究。结果表明,在特定电场条件下,胶体金纳米粒子愈小,其泳动速度愈快,但呈现弥散状分布特征。当胶体金颗粒包敷蛋白分子后即可得到很好的分离,且随标记的蛋白质分子不同而存在很大程度的差异,其在琼脂糖凝胶中的泳动速度依次为BSA,SPA,rhCaM和GAHIgG-γ球蛋白分子。该工作为研究特定生物结构分离、配体分子检测及微器件制作方面提供了新的思路。Several sizes of gold colloidal nanoparticles ranging between5nm and50nm in dia-meter were prepared by using citrate reduction method.Each of them was capped with different proteins,respectively.Elec trophoresis of bar e and proteins-capped gold colloidal nanoparticles through agarose-gel support was carried out.At the same time ,AgNO 3 as well as hydroquinone served as signal enhancer.The samples were well dis played on the plate of agarose-gel,and their position distribution in gel tunn els were also clearly observed.The experimental results showed that,under an a pplied electric field,the smaller size of gold colloidal nanoparticles were,th e faster particles migrated.They were all presented dispersive distribution in agarose-gel.However,the same size of gold colloidal nanoparticles capped wit h proteins were well separated,and their electrophoretic behaviors were analogu es to the characteristic of protein moleculars,but not com-pletely identical with them.Among these particles,the migration speed of which capped with Bovin e serum albumin(BSA)was the fastest,and the order of other capped components was staphylococcal protein A(SPA),recombinant human calmodulin(rhCaM),as well as GAHIgG-? in turn.Furthermore,no dispersion phenomenon occurred.This result provide d a novel idea for separation of biological components,detection of partner mol ecules and preparation of micro/nano structures.

关 键 词：胶体金纳米粒子 蛋白标记物 电场 泳动行为 泳动速度 

分 类 号：TB383[一般工业技术—材料科学与工程]