嗜盐放线菌生物学特性初步研究  

STUDIES ON BIOLOGICAL CHARACTERISTICS OF HALOPHILIC ACTINOMYCETES

作　　者：唐蜀昆[1] 李文均[1] 张永光[1] 徐丽华[1] 姜成林[1] 

机构地区：[1]云南大学省微生物所教育部微生物资源开放研究重点实验室,昆明650091

出　　处：《微生物学通报》2003年第4期15-19,共5页Microbiology China

基　　金：国家科技部基础研究重大项目 (No 2 0 0 1CCC0 0 6 0 0 );云南省自然科学基金项目 (No 2 0 0 1C0 0 0 1Q);云南省教教育厅基金项目 (No 0 1111134 );教育部微生物资源开放研究重点实验室开放基金资助

摘　　要：通过对从新疆 ,青海等地采集的盐碱土样中分离获得的 43株嗜盐或耐盐放线菌以及4株典型菌株的NaCl耐受实验 ,对不同浓度的Na+ ,K+ ,Mg2 + ,Ca2 + 的选择性实验及pH耐受实验进行了研究。发现耐盐放线菌对Na+ ,K+ ,Mg2 + 有广泛的适应性 ,只有少数耐盐放线菌能在较低浓度的CaCl2 条件下生长 ;嗜盐放线菌对Na+ 有广泛的适应性 ,多数嗜盐放线菌生长所需的Na+ 可被K+ ,Mg2 + 所替代 ,而不能被Ca2 + 所替代 ,少数嗜盐放线菌的生长离不开Na+ ,对Na+ 有高度的专一性 ,是专嗜Na+ 的嗜盐放线菌 ,说明不同嗜盐放线菌对Na+ ,K+ ,Mg2 + ,Ca2 + 的适应有选择性。因此 ,提出自然环境中是否也存在类似的专嗜K+ 或专嗜Mg2 + 的嗜盐放线菌的推测。无论是嗜盐还是耐盐放线菌其pH生长范围都在 6 0～1 0之间 ,生长最适pH7 0～ 8 0。另外 。Studies on NaCl concentration, pH tolerance and selectivity to different concent rations of Na +, K +, Mg 2+ and Ca 2+ of 43 isolates from th e saline soils in XinJiang, HeBei and QingHai Provinces of China and 4 type stra ins were performed in this paper Results showed that halotolerant actinomycete s have extensiv e adaptability to Na +, K + and Mg 2+ and only a few of them can grow in low CaCl 2 concentration Halophilic actinomycetes have extensive adaptability to Na +, and for most halophilic actinomycetes, Na + can be substituted by K + , Mg 2+ , but not for Ca 2+ For some halophilic actinomycetes , it is necessary to have Na + for their growth It also showed that the growth of al l halophilic actinomycetes had se lectivity with different concentration of Na +, K +, Mg 2+ So it is pre sumed that only Kaliumphilic or Magnesiumphilic Actinomycetes maybe exist in hig h salt environments In addition, the growth pH range were 6 0～9 0 and the o ptimum pH were 7 0～8 0 not only for halophilic but also for halotolerant acti nomycetes The dis tribution of halophilic actinomycetes also have some relativity to isolation sit es

关 键 词：极端环境 嗜盐放线菌 阳离子 

分 类 号：Q934[生物学—微生物学]