RNA剪接因子结构与功能研究进展  

Structural Analyses of RNA Splicing Factors: Progress and Perspective

作　　者：吴瑛[1,2] 赫荣乔[1] 

机构地区：[1]中国科学院生物物理研究所,视觉信息加工重点实验室 [2]Washington University,School of Medicine,St.Louis,MO 63110,USA

出　　处：《生物化学与生物物理进展》2003年第4期503-508,共6页Progress In Biochemistry and Biophysics

基　　金：国家基金委纳米生物学重大计划重点项目 (90 2 0 60 41);中国科学院创新方向性项目 (KSCX2 SW 2 14 )~~

摘　　要：RNA剪接是一个多步骤、形成多种中间状态复合物的复杂过程 .尽管在已经发现的一百多种 pre mRNA剪接相关因子中 ,仅研究了约 8%相关蛋白质的空间结构 ,已充分显示对剪接相关因子三维晶体结构以及溶液结构的测定与研究 。Pre-mRNA splicing is a critical step in eukaryotic gene expression and regulation. The removal of introns occurs in spliceosome, a macromolecular machine containing small nuclear ribonucleoproteins (snRNP), heterogeneous nuclear ribonucleoproteins (hnRNP) and K-homologous domain (KH) proteins. Crystallographic and NMR studies have begun to provide insight into the architecture of snRNP and the structural basis for RNA-protein as well as protein-protein interactions involved in pre-mRNA splicing. Elucidating molecular mechanisms underlying pre-mRNA splicing awaits systematic structural analyses of splicing factors and complexes.

关 键 词：RNA剪接因子 结构 功能 snRNA结合蛋白 hnRNA结合蛋白 剪接体 晶体结构 

分 类 号：Q756[生物学—分子生物学]