清开灵注射液中三种胆酸含量测定方法比较  

Comparison of three methods for the determination of cholic acids in Qingkailing Injection

作　　者：曹进[1] 徐燕[1] 王义明[1] 罗国安[1] 

机构地区：[1]清华大学生命科学与工程研究院,北京100084

出　　处：《中成药》2003年第9期705-708,共4页Chinese Traditional Patent Medicine

基　　金：国家 973重点基础研究项目"中药方剂关键科学问题基础研究"资助 (课题号G19990 54 40 4 )

摘　　要：目的 :建立同时测定清开灵注射液中 3种胆酸 (牛胆酸 ,猪去氧胆酸和去氧胆酸 )含量的 2种高效液相色谱法(HPLC UV和HPLC ELSD) ,并与原有的测定总胆酸含量的紫外分光光度法进行比较。方法 :高效液相色谱法测定 ,采取紫外与蒸发光散射 2种检测方式 ;结果 :同时测定了清开灵注射液中的 3种胆酸物质 ,方法回收率HPLC/ELSD为1 0 0 % ,HPLC/UV为 97% ,均高于原有的紫外方法 (95 %～ 96 % ) ;结论 :HPLC/ELSD测定清开灵中胆酸成分 ,方法简便准确 。Objective: To establish two methods (HPLC UV and HPLC ELSD)for the determination of 3 kinds of cholic acids in Qingkailing Injection, and compare them with UV method. Methods: Two HPLC methods detected by UV and ELSD, respectively have been developed: Phenomenex Luna C 18 column(250mm×4.6mm,5μ)with linear gradient elution using methanol acetotrile(4∶1) and 0.1% aqueous formic acid. Results: Three kinds of cholic acids in Qingkailing could be determined simultaneously by HPLC UV and HPLC ELSD. The recovery of three method were 100%,97%,95%～96% for HPLC/ELSD,HPLC/UV,UV, respectively. Conclusion: The HPLC ELSD method was accurate and specific to determine the content of cholic acids in Qingkailing Injection.

关 键 词：清开灵 胆酸 蒸发光散射检测 

分 类 号：R927.2[医药卫生—药学]