银杏叶提取物影响臂丛撕脱后运动神经元NOS表达及其存活  

Influence of Ginkgo biloba extract on NOS expression and survival of C7 motoneurons following brachial root avulsion

作　　者：李方澜[1] 周丽华[1] 袁群芳[1] 何宏文[1] 姚志彬[1] 吴武田[2] 

机构地区：[1]中山大学中山医学院解剖教研室,广东广州510080 [2]香港大学医学院解剖系

出　　处：《中国病理生理杂志》2003年第9期1231-1233,T007,共4页Chinese Journal of Pathophysiology

基　　金：广东省科委社会发展攻关项目 (No .2 0 0 2C30 111)

摘　　要：目的 :观察EGb76 1对脊神经根撕脱后前角运动神经元的保护作用和对NOS表达的影响。方法 :成年Sprague -Dawley雌性大鼠 80只 ,行脊髓C5 T1神经根撕脱术后 ,治疗组每天腹腔注射 1mLEGb76 1,2 5mg·kg-1·d-1;对照组注射等量生理盐水。治疗后 1周、2周、4周和 6周分别处死动物 ,取脊髓C7节段行NADPH -d组化和中性红染色 ,计数各组动物损伤侧前角NOS阳性和中性红阳性的运动神经元数目 ,比较组间差异显著性。结果 :脊神经根撕脱后受损运动神经元NOS表达从 1周开始 ,2周达高峰 ,4周 - 6周逐渐下降。运动神经元死亡以 4周 - 6周最明显。EGb76 1能减少以上各时点NOS阳性运动神经元的数目 ,提高各时点运动神经元的存活。结论 :EGb76 1能下调运动神经元NOS基因表达 ,提高受损运动神经元的存活。AIM: To investigate the influence of EGb761 on NOS expression and survival of motoneurons after spinal root avulsion.METHODS: Eighty adult SD female rats were randomly divided into control and EGb761 groups. The C5-T1 nerve roots of right brachial plexus were avulsed and injection of 1 mL of either EGb761 25 mg·kg -1 ·d -1 or normal saline (ip) was performed everyday. The treated rats were killed 1 week, 2 weeks, 4 weeks and 6 weeks following avulsion. The cryostat sections of C7 segment of every rat were collected and carried with NADPH-d histochemistry plus neutral red counterstain. The difference of the numbers of both NOS-positive and survival motoneurons were quantitatively studied. RESULTS: Following avulsion, NOS was expressed in avulsed motoneurons at 1 week, reached to its maximum at 2 weeks and then decreased gradually from 4 weeks to 6 weeks. Motoneurons died from 4 weeks to 6 weeks. With EGb761 treatment, the number of NOS-positive motoneurons were decreased at each time point and the number of survival motoneurons was increased at each time point compared to control rats.CONCLUSIONS: EGb761 protected the spinal cord motoneurons from avulsion injury. This effect may be related to inhibition of NOS expression.

关 键 词：二裂银杏 一氧化氮合酶 运动神经元 臂丛 

分 类 号：R338.2[医药卫生—人体生理学]