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作 者:康强胜[1] 李洪林[1] 吴玉兰[1] 卢大炎[1]
出 处:《天然产物研究与开发》2003年第4期334-336,共3页Natural Product Research and Development
摘 要:新疆紫草细胞生产阶段培养时生物产量随接种量的增加而增大 ,但生物量的倍增数并不增大。色素产量只在一定范围内与接种量成正相关关系 ,超过一定范围反而下降。当选定适当接种量时 ,加大培养基的浓度可提高色素产率。扩大培养规模 ,色素产量下降。This paper reports the effect of inoculum size and medium concentration on the growth and shikonin derivative′s formation in cell culture of Arnebia euchroma .When the amount of inoculum increased,a high biomass yield obtained but cells′ increased-times decreased.The output of shikonm was rose at a range of inoculum size.Exceeded 30 g FW/flask,shikonm formation reduced.Selected a suitable inoculum size,a high medium concentration could result in a high shikonm yield,but the productivity of shikonm decreased with increasing scale.
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