顺铂与亚硒酸钠对人体T淋巴细胞转化及rRNA基因转录活性的影响  

Effects of cisplatin and sodium selenite on transformation and rRNA gene transcription activity of human T lymphocytes

作　　者：陈贤均[1] 赵红刚[2] 

机构地区：[1]咸宁医学院生物学教研室,咸宁437100 [2]咸宁医学院病理学教研室

出　　处：《卫生研究》2003年第4期331-333,共3页Journal of Hygiene Research

基　　金：湖北省教育厅资助 (No .2 0 0 0B4 40 0 11)

摘　　要：为探讨亚硒酸钠对顺铂抑制人体T淋巴细胞转化作用的影响、顺铂与亚硒酸钠对正常人体T细胞rRNA基因转录活性的影响及T细胞转化与rRNA基因表达活性之间的关系 ,在细胞培养 2 4h时加入顺铂(0 0 5、0 2 0和 0 50mg L) ,在细胞培养开始或在加入顺铂的同时加入亚硒酸钠 (0 0 5mg L) ;细胞培养 72h后 ,检测T细胞转化率及Ag NOR表达频率 (反映rRNA基因转录活性 )的变化。结果显示亚硒酸钠与不同剂量的顺铂共同处理淋巴细胞 ,细胞转化率显著高于相应剂量顺铂单独处理组 (P <0 0 1 ) ,并达到或超过阴性对照的水平。亚硒酸钠预先加入或与顺铂同时加入者差异无显著性。各组间Ag NOR的表达频率以及随体联合频率均无显著性差异 (P >0 0 5)。结果表明 ,亚硒酸钠可消除顺铂对T细胞转化的抑制作用 ,但对T细胞转化及对顺铂作用的影响有无时间效应尚不能定论。In order to investigate the effect of sodium selenite on the action of cisplatin inhibiting transformation of human T lymphocytes, explore the influence of cisplatin and sodium selenite on rRNA gene transcription activity of lymphocytes and to explore the relationship between transformation of T cells and rRNA gene transcription activity, cisplatin was administered to the culture of lymphocytes after incubation for 24 hours with three different dosages (0 05, 0 2, and 0 5 mg/L). The culture was also supplemented with sodium selenite (0 05 mg/L) at the beginning of the cell culture or at the same time as cisplatin was administered for different treatment. The slide specimens were prepared using cytogenetical method after the cells were cultured for 72 hours, and then, the changes of the transformation rate of T lymphocytes and frequencies of Ag NORs that reflect rRNA gene transcription activity were investigated. The results showed that the rate of T lymphocyte transformation in the groups treated with both sodium selenite and cisplatin were significantly increased (p<0 01) as compared with that in the groups without sodium selenite supplementation and suffering exposures to cisplatin alone at corresponding dose, up to or over the rate of negative control group. There were no significant differences in the ratios of lymphocyte transformation between the groups pre treated by sodium selenite and the groups treated by both sodium selenite and cisplatin on the condition that the other treatments were the same. There were no remarkable differences (p>0 05) in the frequencies of Ag NOR and satellite association among the all groups. This study indicated that sodium selenite supplementation can preclude the inhibition of tranformation of T lymphocytes in vitro by cisplatin, regardless the pre treatment by sodium selenite or co treatment with cisplatin.

关 键 词：顺铂 亚硒酸钠 T淋巴细胞 RRNA基因 AG-NOR 

分 类 号：R979.1[医药卫生—药品]