小白菊内酯对大鼠血管平滑肌细胞增殖的影响及机制研究  

Sbudies on the Effect of Parthenolide on the Proliferation of Rat Vascular Smooth Muscle Cells and the Mechanism

作　　者：翁少翔[1] 单江[1] 林晓霞[2] 傅国胜[1] 

机构地区：[1]浙江大学医学院附属二院,浙江杭州310009 [2]浙江大学医学院附属邵逸夫医院,浙江杭州310009

出　　处：《中国中药杂志》2003年第7期647-650,共4页China Journal of Chinese Materia Medica

摘　　要：目的:探讨小白菊内酯对血管平滑肌细胞增殖的影响及作用机制.方法:培养大鼠胸主动脉血管平滑肌细胞(VSMC),Western印迹杂交法检测c-fos, c-myc, p15, p16, p18, p19蛋白表达,流式细胞仪检测VSMC周期情况,[3H]TdR参入测定VSMC 的DNA合成.结果:小白菊内酯以时间依赖关系抑制c-fos, c-myc蛋白表达,但不影响p15, p16, p18, p19蛋白表达,以剂量依赖关系使VSMC周期中的G0/G1期细胞比例明显增多,S期细胞比例显著减少,同时抑制VSMC的[3H]TdR参入.结论:小白菊内酯可能通过抑制c-fos, c-myc蛋白表达,而不是影响p15, p16, p18, p19蛋白表达来抑制VSMC增殖.Objective: To study the effect of parthenolide on the proliferation of vascular smooth muscle cell(VSMC) and its mechanism. Method: Vascular smooth muscle cell was cultured, the protein levels of c-fos, c-myc, p15, p16, p18, p19 were measured by Western blot method, cell cycle were examined with flow cytometry, and the DNA synthesis was determined by [3H]-TdR incorporation. Result: Parthenolide inhibited protein levels of c-fos, c-myc in a time-dependent manner but didn't affect the protein levels of p15, p16, p18, p19. Flow cytometric DNA analysis revealed that parthenolide increased significantly G0/G1 phase of VSMC and decreased S phase of VSMC in a dose-dependent manner. Parthenolide inhibited [3H]-TdR incorporation in a dose dependent manner. Conclusion: Parthenolide may inhibit proliferation of VSMC by inhibiting the expressions of c-fos, c-myc, but not the expressions of p15, p16, p18, p19.

关 键 词：血管平滑肌细胞 细胞增殖 小白菊内酯 C-FOS C-MYC 基因表达 动物实验 

分 类 号：R285.5[医药卫生—中药学]