Schwann细胞转染Ha-Ras对 β_1 ,4-半乳糖基转移酶I,II,V表达的影响  

Changes of β-1,4-galactosyltransferase I,II and V in Schwann cells tranfected with oncogene Ha-Ras

作　　者：沈爱国[1,2] 丁斐[1] 强亮[1] 龚蕾蕾[1] 王汉洲[2] 顾建新[2] 

机构地区：[1]江苏省神经再生重点实验室,南通医学院,江苏南通226001 [2]复旦大学上海医学院基因研究中心,上海200032

出　　处：《中国神经科学杂志》2003年第4期242-246,共5页

基　　金：江苏省神经再生重点实验室开放课题基金项目 (JSK0 2 0 12 )

摘　　要：目的 :研究原代Schwann细胞转染持续激活型原癌基因Ha Ras后β1,4 半乳糖基转移酶I,II ,V(β 1,4 galactosyltransferaseI ,II ,V ;β 1,4 GalT I,II,V)表达变化。方法 :构建Ha Ras表达质粒和分离纯化大鼠Schwann细胞 ,将Ha Ras表达质粒转染到Schwann细胞中。采用NorthernBlot方法分析转染细胞中Ha Ras转染情况和转染后β 1,4 GalT I,II,V的表达变化。结果 :原代Schwann细胞转染持续激活型Ha Ras后 ,β 1,4 GalT I随转染时间延长而表达增高 ,而 β 1,4 GalT II及V的表达无变化。 结论 :β 1,4 GalT I随Schwann细胞转染时间Ha Ras延长而表达增高 ,提示原代细胞表面的 β 1,4 GalT I可能与Ha Ras影响原代细胞增殖的信号途径相关。Objective:To investigate the changes of β 1,4 galactosyltransferase I,II and V(β 1,4 GalT I,II and V) in Schwann cells transfected with oncogene Ha Ras. Methods: The constructed expression plasmids of oncogene Ha Ras were transiently tranfected into purified Schwann cells by liposomes. The changes of β 1,4 GalT I,II and V in Schwann cells transfected with the expression plasmids of Ha Ras was studied by Northern Blot. Results: The expression of β 1,4 GalT I mRNA was increased in Schwann cells transfected with Ha Ras while those of β 1,4 GalT II and V mRNA had no obvious change. Conclusion: It is suggested the β 1,4 GalT I of cell surface may associate with the path of influencing cell proliferation by oncogene Ras. \[

关 键 词：Schwann细胞转染 HA-RAS β1 4-半乳糖基转移酶Ⅰ 质粒转染 原癌基因 β-1 4-GalT-Ⅱ β-1 4-GalT-Ⅴ 表达 

分 类 号：R341[医药卫生—基础医学]