口蹄疫病毒融合表位基因在pET原核系统的高表达及产物的纯化  

High expression of fusion epitopes cDNA of foot-and-mouth disease virus in pET proeukaryotic system and purification of products

作　　者：陈祖欢[1] 张洪英[1] 郭瀛军[1] 林懿[1] 朱维佳[1] 孙树汉[1] 

机构地区：[1]第二军医大学基础医学部医学遗传学教研室,上海200433

出　　处：《第二军医大学学报》2003年第8期904-905,共2页Academic Journal of Second Military Medical University

基　　金：国家"8 63"计划资助项目 ( 2 0 0 1AA2 13 111)

摘　　要：构建口蹄疫融合表位基因 - SG表达载体 p ET- SG,转化重组子与 K80 2 ,新鲜过夜菌以 2 %接种量接种 2 YT培养液 ,37℃培养 2 h后用 IPTG诱导 ,每隔 1h取样 ,共 8h。根据 SDS- PAGE结果 ,口蹄疫融合表位基因 - SG在 p ET原核表达系统可获得较高表达 ,用 IPTG诱导 4 h蛋白表达量最高 ,用 Ni+柱亲和层析可得到较纯蛋白。Proeukaryotic expression vector pET SG for fusion epitopes of foot and mouth disease virus(FMDV) was constructed and transferred into K802.Fresh bacterium was added to 2YT culture medium at a radio of 2% and the system was incubated for 2 h and then induced by IPTG.Expression products was collected every hour and the best expression time was decided by SDS PAGE.FMDV epitope genes SG was expressed with high yield in pET proeukaryotic system,K802 pET SG produced the largest amout of fusion protein when induced by IPTG for 4 h, and pure fusion proteins was obtained through a Ni + affinity chromatography column.

关 键 词：口蹄疫病毒 融合表位基因 pET原核系统 基因表达 纯化 

分 类 号：S852.659.6[农业科学—基础兽医学]