头孢克罗与人血清白蛋白相互作用机制  

The Reaction Mechanism between Cefaclox and Human Serum Albumin

作　　者：刘洛生[1] 赵丽[2] 葛蔚颖[2] 张虞毅[3] 

机构地区：[1]山东大学化学与化工学院,山东济南250012 [2]山东大学药学院,山东济南250012 [3]山东大学医学院,山东济南250012

出　　处：《光谱学与光谱分析》2003年第4期769-771,共3页Spectroscopy and Spectral Analysis

摘　　要：以光谱技术研究了人血清白蛋白 (HSA)与头孢克罗分子间结合作用机制。由Lineweave Burk双倒数作图法确定该反应的解离常数 Kd =1 2 2× 10 -4,用同步荧光技术考察了头孢克罗对人血清白蛋白物象的影响。依据Forster非辐射能量转移机制 ,得到给体 受体间的结合距离和能量转移效率。头孢克罗与人血清白蛋白以疏水作用为主。抗生素效力与能量转移效率和解离常数有关。To study the reaction mechanism between cefaclox and human serum albumin(HSA) in aqueous solution, fluorescence spectra were used. The interaction dissociation constants of human serum albumin and cefaclox were determined from a double reciprocal Lineweaver-Burk plot. The effect of cefaclox on the conformation of HSA was analyzed using synchronous fluorescence spectrometry. The binding distance and transfer efficiency between cefaclox and HSA were also obtained according to the theory of Forster non-radiation energy transfer. The main binding force between cefaclox and HSA is hydrophobic interaction. The antibiotic valence is related to the transfer efficiency and dissociation constant.

关 键 词：头孢克罗 人血清白蛋白 相互作用 作用机制 Β-内酰胺类抗生素 荧光法 

分 类 号：R978.11[医药卫生—药品] R331.1[医药卫生—药学]