微流控芯片操纵传输及实时监测单细胞量子释放  

Manipulation,Transport and Quantal Release Monitoring of Single Cells on a Microfluidic Device

作　　者：程伟[1] 黄卫华[1] 庞代文[1] 王宗礼[1] 程介克[1] 崔大付[2] 屈三甫[3] 郑从义[3] 

机构地区：[1]武汉大学化学与分子科学学院分析科学中心,武汉430072 [2]中国科学院电子研究所传感技术国家重点实验室,北京100080 [3]武汉大学生命科学学院,武汉430072

出　　处：《高等学校化学学报》2003年第9期1585-1587,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20299034)

摘　　要：微流控芯片技术用于细胞生化分析已引起了广泛关注.A novel microfluidic device was developed for on-chip manipulation and monitoring exocytosis of single cells. The microfluidic device consists of a top layer of PDMS containing channels for introducing cells and stimulants and a bottom glass layer into which a cell microchamber was etched. By using hydraulic pressure , single cells were transported and located on the microfluidc chip. After the location of single PC12 cells on the microfluidic chip, the cell was stimulated with nicotine that was also introduced through the microchannels, and the quantum release of dopamine from the cell was detected by ampero-metric determination with our designed carbon fiber microelectrode. The results demonstrate the convenience and efficiency of using the microfluidic chip for monitoring of single cells, and offered a new profile for the analysis of single cells on the microfluidic devices.

关 键 词：微流控芯片 单细胞操纵 实时监测 单细胞活体 量子释放 碳纤维微电极 生物化学分析 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]