青枯无致病力菌株诱导番茄抗青枯病的生化机制  

Biochemical mechanism of induced resistance to tomato bacterial wilt with the treatment of avirulent strains of Ralstonia solanacearum

作　　者：陈庆河[1] 翁启勇[1] 胡方平[2] 

机构地区：[1]福建省农科院植保所,福建福州350013 [2]福建农林大学植保学院,福建福州350003

出　　处：《福建农林大学学报（自然科学版）》2003年第3期296-300,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省自然科学基金资助项目(B0210031);福建省科技厅重大项目(2000Z-006).

摘　　要：用紫外诱变法获得的青枯无致病力菌株诱导番茄,植株产生了对青枯病的抗性反应,该菌株对致病菌没有直接的抑制作用,且对番茄不能致病.番茄经无致病力菌株处理后,体内与抗病反应相关的苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)及多酚氧化酶(PPO)活性显著增强;酚类物质和木质素含量也显著提高;病程相关蛋白(PRP)含量也提高.这表明青枯无致病力菌株产生诱导抗性的机制可能是植物本身的抗病代谢过程被激活了.Avirulent strains of Ralstonia solanacearum (ATm044 and ASp061) obtained by means of UVray mutation produced induced resistance to tomato bacterial wilt with the effect of 59.1% or 53.4%, which had no inhibiting effect on virulent strains, and could not become pathogeny of tomato. After the application of the strains, the activities of peroxidase(POD), phenylanine ammonia lyase(PAL) and polyphenoloxidase(PPO) in tomatos were increased significantly, and the contents of phenolic compounds and lignin in tomatos also markedly increased; the contents of pathogenesisrelated protein(PRP) were also increased. The results indicated that the mechanism of induced resistance to tomato bacterial wilt may be the changes in metabolism related to the resistance.

关 键 词：番茄 青枯病 诱导抗病性 无致病力菌株 青枯病菌 生化机制 抗病代谢过程 苯丙氨酸解氨酶 过氧化物酶 多酚氧化酶 酚类物质 木质素 病程相关蛋白 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]