腹部创伤早期脑组织及血清神经元特异性烯醇化酶的改变(英文)  

Early changes of neuron-specific enolase in serum and brain tissue after explosive abdomen wounds in dogs

作　　者：陈向阳[1] 李兵仓[1] 张良潮[1] 陈菁[1] 吴国萍[1] 

机构地区：[1]解放军第三军医大学大坪医院野战外科研究所第六研究室,重庆市400042

出　　处：《中国临床康复》2003年第22期3077-3078,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的观察腹部爆炸伤后早期脑组织、血清神经元特异性烯醇化酶(neuron-specificenolase,NSE)的变化规律,以探讨血清NSE对这种间接性脑损伤的早期诊断价值。方法健康雄性杂种犬22只,随机分为正常对照组5只,致伤组17只。制作犬腹部爆炸伤动物模型。伤后测定不同时相点血清神经元特异性烯醇化酶浓度,并于相应时相点观察皮层神经元NSE免疫组织化学反应强度及反应阳性细胞数目的变化。结果伤后即刻血清NSE水平达11.6μg/L,显著高于伤前8.2μg/L(F=26.29,P<0.01),伤后1.5h达峰值16.9μg/L,12h以后迅速下降,到伤后24h为9.6μg/L,基本接近伤前水平(P>0.05)。皮层神经元NSE免疫组织化学反应强度伤后即刻为164灰度级,明显低于正常对照组178灰度级(F=10.37,P<0.01),且持续下降,伤后24h为161灰度级,仍显著低于正常对照组(F=45.23,P<0.01)。正常皮层NSE免疫组织化学染色阳性细胞计数为43个,伤后6h下降为35个,两者相差显著(F=6.99,P<0.01),伤后24h为29个,与正常对照组比相差仍然显著(F=20.90,P<0.01)。结论血清NSE水平的检测可作为腹部爆炸伤后脑损伤的早期诊断指标之一。AIM:To observe early changes of neuron-s pecific enolas e (NSE)in serum and brain tissue after explosive abdomen wounds so as to provide useful parameter for early diagnosis.METHODS:Twenty two healthy male hybridi zed d ogs were divided ran-domly into control group(n=5)and injury group(n =17).Dog model of explosive abdomen injury was establ ished.Levels of neur on-specific en olase(NSE)in the serum were determined at diffe rent phases.M eanwhile,the numbers of NSE immunohistochemical reactive positive cells and th e reac tive intensity in the cortex were observe d.RESULTS:NSE level reached 11.6μg /L at instant time after injury whic h was significantly higher tha n pre-injury(F=26.29,P<0.01),and reached the peak at 1.5hour after inj ury.Then,the level of serum-NSE began to drop dramatically from 1 2hours after injury and almost reached t he preinjury level (9.6μg /L). The intensity of NSE immunohistoch emical re-action in the cortex(164gray g rade)at instant time after injury was sign if-icantly decreased than control g rou p(178gray grade,F=10.37,P<0.01),and decreased continuousl y.The gray grade at 24h after injury was161which was also lower than contr ol group(F=45.23,P<0.01).The numbers of NSE positive cells in th e c ortex(35)were obviously decreased till6hours after injury(F=6.99 ,P<0.01),and kept this tendency to 24hours after injury(29)(F=2 0.90,P<0.01).

关 键 词：腹部创伤早期 脑组织 血清 神经元特异性烯醇化酶 腹部爆炸伤 间接性脑损伤 

分 类 号：R656[医药卫生—外科学] R651.15[医药卫生—临床医学]