抗猪口蹄疫病毒单克隆抗体的抗原识别位点分析  

Analysis of the Antigenic Epitopes Recognized by Monoclonal Antibody against Foot and Mouth Disease Virus(FMDV)

作　　者：何永强[1] 盛祖恬[1] 杜青云[1] 倪征[1] 

机构地区：[1]浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021

出　　处：《中国兽医学报》2003年第5期433-434,共2页Chinese Journal of Veterinary Science

基　　金：浙江省自然科学基金资助项目 (3 970 45 ) ;国家科委九五"863"项目

摘　　要：用 EL ISA叠加试验 ,对 5株具有 EL ISA反应特性的抗猪口蹄疫病毒 (FMDV)单克隆抗体 (Mc Ab- A6、F9、G17、G5 2、S2 5 )的抗原识别位点进行检测。抗体反应增值结果表明 ,5株单抗分别针对 4个不同抗原位点 ,Mc Ab- G17、G5 2、S2 5识别的位点各不相同 ,其中 Mc Ab- G7、G5 2识别的位点有部分重叠 ,Mc Ab- A6、F9识别同一位点 ,位于 G17、G5 2位点的重叠区内。Antigenic epitopes recognized by five strains of monoclonal antibodies(McAb A6,F9,G17,G52,S25) with ELISA reaction characteristics against foot and mouth disease virus(FMDV) were detected by means of competitive binding assay.The result indicated that the five strains of McAbs were specific for four different kinds of epitopes on FMDV.Among them,McAb G17,McAb G52 and McAb S25 direct to different epitopes,respectively.The epitope recognized by McAb G17 overlapped partly that of McAb G52.McAb A6 was identical to McAb F9.The epitope of which was supposed to be locateed in the overlapping region between epitope G17 and epitope G52.

关 键 词：猪口蹄疫病毒 单克隆抗体 抗原位点 ELISA叠加试验 

分 类 号：S858.28[农业科学—临床兽医学] S852.659.6[农业科学—兽医学]