小鱼仙草总黄酮提取工艺的初步研究  

A Preliminary Study on Total Flavonoids from Mosla dianthera

作　　者：刘小芬[1] 林文群[1] 刘剑秋[1] 陈炳华[1] 

机构地区：[1]福建师范大学生物工程学院,福建福州350007

出　　处：《福建师范大学学报（自然科学版）》2003年第3期118-120,共3页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省科技厅基金资助项目(K20096)

摘　　要：利用聚酰胺—硝酸铝显色法测定不同产地小鱼仙草(茎、叶)总黄酮含量.对不同酒精体积分数、提取倍量、提取温度及提取时间等条件进行了初步分析.结果表明,40倍量、75%乙醇于80℃恒温中提取3h为最佳条件.在此条件下,1号小鱼仙草总黄酮含量为8.67%,2号小鱼仙草总黄酮含量为12.46%,3号小鱼仙草总黄酮含量为3.16%.This paper reports the studying on total flavonoids from stem and leaf of Mosla dianthera Maxim which grow in different circumstance, extracted with different extraction conditions, purified by polycaprolactan and determined. Results showed that the best way is extracted by 75% ethanol in 80℃ for at least 3 hours. The maxim content of the total flavonoids is 867% in No1,1246% in No2 and 316% in No3 respectively.

关 键 词：小鱼仙草 总黄酮 提取工艺 聚酰胺-硝酸铝显色法 体积分数 提取倍量 提取温度 提取时间 唇形科 中药 

分 类 号：R284.2[医药卫生—中药学] Q946.83[医药卫生—中医学]