精甲霜灵与外消旋体甲霜灵对掘氏疫霉菌的抑菌活性比较  

The Comparison of Inhibitory Action between Stereoisomers of Metalaxyl

作　　者：刘西莉[1] 马安捷 林吉柏[1] 刘鹏飞[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094 [2]北京清新泰和运动草坪科技有限公司,北京100029

出　　处：《农药学学报》2003年第3期45-49,共5页Chinese Journal of Pesticide Science

基　　金：农药化学及农药使用技术农业部重点开放实验室开放基金资助项目.

摘　　要：采用生长速率法测定精甲霜灵、外消旋体甲霜灵及其混配药剂对掘氏疫霉菌PhytophthoradrechsleriTucker的杀菌毒力。应用不同药剂浓度的白云豆液体和固体带毒培养基培养法及玻璃纸培养法测定甲霜灵、精甲霜灵对病原菌菌丝生长、孢子囊萌发和游动孢子产生的影响,采用电导法测定药剂对病原菌细胞膜通透性的影响。实验结果表明,精甲霜灵和外消旋体甲霜灵对掘氏疫霉菌的EC50值分别为0.129μg/mL和0.427μg/mL;两者分别与代森锰锌按照质量比5∶48的配比混配,共毒系数分别为101.6和95.9,对防治由掘氏疫霉菌引起的西瓜疫病具有加和作用。甲霜灵和精甲霜灵对病原菌菌丝的生长和孢子囊的产生都有很高的抑制活性,当药剂浓度为0.3～0.7μg/mL时,精甲霜灵对菌丝生长的抑制率为39.28%～84.39%,明显优于甲霜灵的28.36%～66.56%。药剂浓度为30.0～70.0μg/mL时,精甲霜灵对孢子囊产生的抑制率为42.7%～98.3%,优于甲霜灵的25.1%～89.5%。两种药剂对孢子囊的萌发没有明显影响;精甲霜灵对病原菌细胞膜通透性的影响高于甲霜灵。Toxicity of metalaxylM, metalaxyl and their mixtures to Phytophthora drechsleri were determined by means of growth rate, respectively. Effect of metalaxylM and metalaxyl on hypha growth, sporangium germination, swarm sporangium formation were examined by the ways of liquidsolid white lupine media mixed with fungicide of different concentration. The result indicated that the EC50 of metalaxylM and metalaxyl were 0.129 μg/mL and 0.427 μg/mL . The common toxicity index of mixture 1 containing metalaxylM and mancozeb (5∶48, m/m) and mixture 2 containing metalaxyl and mancozeb (5∶48, m/m) were 101.6 and 95.9, respectively. MetalaxylM and mixture 1 showed more effective toxicity to Phytophthora drechsleri than that of metalaxyl and mixture 2. MetalaxylM had higher inhibitory action on hypha growth and sporangium formation than that of metalaxyl under the same concentration. When the concentration was 0.3～0.7 μg/mL and 30.0～70.0 μg/mL respectively, the inhibition rate of metalaxylM and metalaxyl to the growth of hypha were 39.28%～84.39% and 28.36%～66.56%, to sporangium formation were 42.7%～98.3% and 25.1%～89.5%, but metalaxylM or metalaxyl had no inhibitory activity to sporangium germination. Effect of fungicides on the cell membrance potential of Phytophthora drechsleri was tested by the conductivity method, which reveal that metalaxylM made a significant infection on cell membrance than that of metalaxyl.

关 键 词：精甲霜灵 手性异构体 毒力测定 抑菌作用 

分 类 号：S482.2[农业科学—农药学]