典型光活化毒素α-三噻吩对棉铃虫和亚洲玉米螟谷胱甘肽S-转移酶的影响  

Effect of α-Terthienyl on Glutathione S-transferases in Helicoverpa armigera and Ostrinia furnacalis Larvae

作　　者：蒋志胜[1] 颜增光[1] 杜育哲[2] 尚稚珍[1] 

机构地区：[1]南开大学元素有机化学研究所元素有机化学国家重点实验室,天津300071 [2]南开大学生命科学学院生物系,天津300071

出　　处：《农药学学报》2003年第3期76-79,共4页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金(39900094);天津市自然科学基金(003701311)资助课题.

摘　　要：研究了典型光活化毒素α-三噻吩(简称α-T)对棉铃虫Helicoverpaarmigera和亚洲玉米螟Ostriniafurnacalis谷胱甘肽S-转移酶(GSTs)的影响。结果表明:近紫外光照(300～400nm)对棉铃虫和亚洲玉米螟幼虫GSTs活性无显著影响,但两种昆虫GSTs对α-T的反应不同。无光照条件下,α-T对亚洲玉米螟离体GSTs活性没有影响,而高浓度α-T能抑制棉铃虫离体GSTs活性;高剂量α-T可使两种昆虫活体GSTs活性升高。光照条件下,高浓度和高剂量α-T抑制棉铃虫GSTs活性,而不影响亚洲玉米螟离体GSTs活性,但低剂量α-T抑制其活体活性,而高剂量α-T则诱导其活性增加。棉铃虫和亚洲玉米螟GSTs对α-T反应的差异,可能与它们对药剂的敏感性以及药剂在两者体内的穿透、运转、贮藏、代谢等生理特性上的差异有关。The effects of αterthienyl (αT) on the glutathione Stransferases(GSTs) were investigated in Helicoverpa armigera and Ostrinia furnacalis. Results indicated that near ultraviolet irradiation (300~400 nm) had no effects on GSTs activities in the control larvae of H. armigera and O.furnacalis both in vitro and in vivo. Treatment with high concentration of αT inhibited the GSTs in vitro in H. armigera, but had no effects in O.furnacalis. Following topical administration, high doses of αT increased the GSTs activities in vivo both in two insects. Upon ultraviolet irradiation, high concentration of αT decreased the GSTs activities in vitro in H. armigera, But had no effects in O.furnacalis. After topical application, high dose of αT reduced the activity in vivo in H. armigera but induced it in O.furnacalis. The differential response of GSTs against αT in H. armigera and in O.furnacalis may be interpreted by their different sensitivities to αT. And the variances in penetration, transportation, storage and metabolism of αT may be also contributed to the differentiation.

关 键 词：α-三噻吩 棉铃虫 亚洲玉米螟 谷胱甘肽S-转移酶 

分 类 号：Q965[生物学—昆虫学]