水稻株高、抽穗期和有效穗数的QTL与环境的互作分析  

Analysis of Additive and AE Interaction Effects of QTLs Controlling Plant Height,Heading Date and Panicle Number in Rice (Oryza sativa L.)

作　　者：袁爱平[1] 曹立勇[1] 庄杰云[1] 李润植[2] 郑康乐[1] 朱军[3] 程式华[1] 

机构地区：[1]中国水稻研究所国家水稻改良中心,杭州310006 [2]山西农业大学生物工程中心,太谷030801 [3]浙江大学农业与生物技术学院,杭州310029

出　　处：《Acta Genetica Sinica》2003年第10期899-906,共8页

基　　金：浙江省重点项目 (0 10 0 0 70 15 );国家 863计划 (2 0 0 1AA2 110 81);农业部水稻生物学重点实验室开放项目~~

摘　　要：株高、抽穗期和有效穗数是水稻的重要农艺性状 ,合适的株高、抽穗期和有效穗数对水稻的高产稳产是至关重要的。该实验应用中 15 6 谷梅 2号的重组自交系 (RIL)群体 ,建立由 16 8个DNA分子标记组成的遗传连锁图 ,以一年两季作为不同的环境效应 ,对水稻株高、抽穗期和有效穗数进行了非条件和条件QTL定位 ,在非条件QTL定位中共检测到 7个株高QTLs、5个抽穗期QTLs和 3个有效穗数QTLs和 10对加加上位性互作位点 ,条件QTL定位结果表明 ,抽穗期这一性状对株高和有效穗数QTLs的表达既有抑制作用 。Plant height (PH),heading date (HD) and producti ve panicle number (P N) are important agronomic traits in rice.Appropriate plant height,heading date and panicle number are prerequisites for the desired high and stable yield leve l in rice breeding programs.A recombinant inbred line (RIL) population consisti ng of 304 individuals was derived from a cross between indica varieties Zhong156 and Gumei2,from which a linkage map consisting of 168 RFLP,SSLP,RAPD and RG A markers that distribute on all the 12 rice chromosomes was constructed,and co vers 1447.9 cM of the rice genome. The parents and 304 F 9 lines were grown in the paddy field in China National R ice Research Institute (CNRRI),Hangzhou,China in 2001.The experiments were carr ied out in two seasons followed a randomized complete block design.QTLMapper 1. 01 was applied to detect QTLs and QTL × environment (QE ) interaction for HD (h eading data),PH (plant height) and PN (panicle number),and conditional mapping for PH and PN was performed as well. A total of 15 QTLs with significant additive effects were detected,among which 4 QTLs had significant QE interaction.Ten QTLs with additive × additive ep istatic effects for PH,HD and PN were detected,among which none showed significa nt epistatisis × environment interaction.These QTLs explained 12.12%,1.38% an d 5.00% of the total phenotypic variance for PH,HD and PN,respectively,and c ontributions were generally lower due to the strong epistatic effects. In conditional QTL analysis,the numbers of QTLs showing significant additive an d epistatic effects were 7 and 6 for PH,and 3 and 3 for PN,respectively.Among the QTLs having significant additive effects for PH,qPH7-2 showed both additiv e effects and QE interaction,qPH7-1 and qPH10 showed QE interaction only,and the remaining 4 QTLs showed additive effec ts only.Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction.No epistatic QE interactions was dete cted.In addition,conditional QTL analysis indicated that

关 键 词：数量性状基因(QTL) 非条件QTL定位 条件QTL定位 加性效应 上位性互作效应 

分 类 号：S511[农业科学—作物学]